Comparative study of Argania spinosa seed oil by 1H NMR spectroscopy and mass spectrometry

S.V. Goryainov; Zakaria Labzioui; A.M. Aliev; S.N. Suslina; V.V. Vandishev; G.A. Kalabin

Comparative study of Argania spinosa seed oil by 1H NMR spectroscopy and mass spectrometry

S.V. Goryainov¹, Zakaria Labzioui², A.M. Aliev³, S.N. Suslina⁴, V.V. Vandishev⁵, G.A. Kalabin⁶

research article Pages: 10-15

¹ зав. лабораторией масс-спектрометрии высокого разрешения и спектроскопии ЯМР, ЦКП (НОЦ), Российский университет дружбы народов (Москва)
² аспирант, кафедра общей фармацевтической и биомедицинской технологии, Российский университет дружбы народов
³ науч. сотрудник, Горный ботанический сад, Дагестанский научный центр РАН (г. Махачкала)
⁴ к.фарм. н., доцент, кафедра общей фармацевтической и биомедицинской технологии, Российский университет дружбы народов
⁵ к.фарм.н., доцент, кафедра ботаники, физиологии растений и агробиотехнологии, Российский университет дружбы народов
⁶ д.х.н., профессор, директор, Центр прецизионных инструментальных методов анализа, ЦКП (НОЦ), Российский университет дружбы народов

Keywords: argan oil seeds comparative study lipid-lowering effect

Abstract

The aim of the work was a comparative study of Argania spinosa seeds oil samples obtained in the traditional way - by hand, as well as by methods of Soxhlet extraction with n-hexane and supercritical fluid CO2 extraction for the development of approaches to the authentication and purity of argan oil.

Argania spinosa seeds oil is a valuable product for the cosmetics and pharmaceutical industries. With the help of precision physico-chemical methods - 1H NMR spectroscopy and mass-spectrometry - in comparative terms set triglyceride, fatty acid and sterols composition, as well as the composition of Tocopherols and the content of squalene in the samples of oil арганы of different origin. Low content of kampesterol among sterols authentic sample oil арганы can be used as an indicator of the feature in establishing the authenticity of Argania spinosa seeds oil.

How to cite

Горяинов С.В., Лабзиуи З., Алиев А.М., Суслина С.Н., Вандышев В.В., Калабин Г.А. Сравнительное исследование образцов масла семян Argania spinosa, полученных разными способами, методами ЯМР 1Н спектроскопии и масс-спектрометрии // Вопросы биологической, медицинской и фармацевтической химии. 2013. № 4. С. 10–15.

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[1] Tuteljan V.A., EHller K.I. Metody analiza minornykh biologicheski aktivnykh veshhestv pishhi. M. 2010.

[2] Khallouki F., Mannina L., Viel S., Owen R.W. Thermal stability and long-chain fatty acid positional distribution on glycerol of argan oil // Food Chemistry. 2008. V. 110. P. 57-61.

[3] Matthaus B., Guillaum D., Gharby S., Haddad A., Harhar H., Charrouf Z. Effect of processing on the quality of edible argan oil // Food Chemistry. 2010. V. 120. P. 426-432.

[4] Hilali M., Charrouf Z., Soulhi A.E.A., Hachimi L., Guillaume D. Detection of Argan Oil Adulteration Using Quantitative Campesterol GC-Analysis // J. Amer. OilChem. Soc. 2007. V. 84. P. 761-764.

[5] CodexAlimentarius.ZHiry, masla i proizvodnye produkty. M.: Izd-vo Ves mir. 2007.

[6] CHernecova E.S., Morlok G.E., Revelskijj I.A. Mass-spektrometrija DARTi ee primenenie v khimicheskom analize // Uspekhi khimii. 2011. T. 80. № 3. S. 249-271.

[7] Khallouki F., Younos C., Soulimani R., Oster T., Charrouf Z., Spiegelhalder B., Bartsch H., Owen R.W. Consumption of argan oil (Morocco) with its unique profile of fatty acids, tocopherols, squalene, sterols and phenolic compounds should confer valuable cancer chemopreventive effects // European Journal of Cancer Prevention 2003. V. 12. P. 67-75.