Е.И. Антонова1, Н.В. Фирсова2, Н.А. Ленгесова3

1–3 Научно-исследовательский центр фундаментальных и прикладных проблем биоэкологии и биотехнологии ФГБОУ ВО «Ульяновский государственный педагогический университет им. И.Н. Ульянова» (г. Ульяновск, Россия)

1 antonov_67@mail.ru, 2 n-firsova@mail.ru, 3 lengesova@yandex.ru

Постановка проблемы. Для эффективного производства целевых белков доступны различные системы экспрессии, включая прокариотические, эукариотические и системы млекопитающих. Pichia pastoris (теперь Komagataella phaffii) является наиболее широко используемой экспрессионной системой в области генной инженерии как более эффективная система экспрессии целевого белка. Широкая сфера практического применения P. pastoris в качестве экспрессионной системы определяет необходимость более глубокого морфофункционального, генетического и филогенетического анализа данной группы микроорганизмов.

Цель работы – проведение ретроспективного мета- и библиометрического анализа в формате «scoping review» (аналитический обзор) опубликованных экспериментальных и фундаментальных исследований относительно истории использования в качестве экспрессионной системы, таксономического положения, организации генома, метаболизма метилотрофных дрожжей P.pastoris (Komagataella phaffii) в сравнении с другими системами экспрессии и сферы практического применения.

Результаты. Представлены систематизированные данные биологии метилотрофных дрожжей P.pastoris (Komagataella phaffii). В частности, рассмотрено филогенетическое положение микроорганизма, использование P.pastoris в качестве организма-продуцента рекомбинантных белков в процессе биотехнологического производства, представлены все виды штаммов и детальный анализ организации генома, а также промоторов и плазмид. Рассмотрены метаболические пути P.pastoris.

Практическая значимость. Данный обзор представляет собой системный и глубокий анализ биологии P.pastoris (Komagataella phaffii) как для области фундаментальных, так и для сферы практического применения информации, изложенной в статье. Обзор включает в себя новые данные биологии P.pastoris (Komagataella phaffii), дает возможность получить развернутое и систематизированное представление по данному вопросу, поскольку одной из главных задач современного исследователя является поиск релевантной информации, обладающей научной ценностью.

Антонова Е.И., Фирсова Н.В., Ленгесова Н.А. Характеристика Pichia pastoris (Komagataella phaffii) как экспрессионной системы для биотехнологического производства // Технологии живых систем. 2024. T. 21. № 4. С. 112-129. DOI: https://doi.org/10.18127/ j20700997-202404-12

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