А.В. Комин1, Н.С. Лешукова2

1,2 ФГБОУ ВО Ярославский государственный технический университет (г. Ярославль, Россия)

1 kominav@ystu.ru, 2 leschukovan@yandex.ru

Постановка проблемы. Одним из стандартных видов исследований новых полимерных материалов и изделий из них медицинского назначения, предполагающих контакт с кровью, является тест на гемолиз. В настоящее время существуют различные подходы изучения гемолитических свойств полимерных материалов in vitro. Ввиду отсутствия единой стандартизированной методики теста на гемолиз исследователи по-разному его реализуют. В связи с этим возникает вопрос не только в достоверности результатов исследований, но и в возможности их сравнения.

Цель работы – анализ и обобщение мирового опыта оценки гемолитической активности полимерных материалов in vitro.

Результаты. Приведен анализ существующих подходов к оценке гемолитических свойств полимерных материалов. Показаны преимущества прямых методов исследования, предполагающие прямой контакт исследуемого материала с суспензией эритроцитов. Проанализированы и обобщены методические аспекты, включая выбор доноров крови, подготовки суспензии эритроцитов и образцов материала, время контакта материала с эритроцитами, условия центрифугирования и измерения характеристик системы, расчета и оценки результатов.

Практическая значимость. Настоящее исследование может служить научной основой для разработки новой, относительно простой и универсальной методики оценки гемолитических свойств полимерных материалов и изделий in vitro медицинского назначения.

Комин А.В., Лешукова Н.С. Оценка in vitro гемолитического действия полимерных материалов и изделий для использования в медицине // Технологии живых систем. 2024. T. 21. № 4. С. 80-92. DOI: https://doi.org/10.18127/j20700997-202404-09

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