A.V. Komin1, N.S. Leshukova2

1,2 FGBOU VO Yaroslavl State Technical University (Yaroslavl, Russia)

1 kominav@ystu.ru, 2 leschukovan@yandex.ru

The paper is devoted to the review of methods for studying the hemolytic action of polymeric materials and products made of them in-vitro. Classification and discussion of different methods are given. The main emphasis is made on the method involving direct contact of the material with blood. The issues of blood donor selection, its preparation for analysis, preparation of test samples, positive and negative control samples, conditions of erythrocyte suspension preparation, sample incubation, centrifugation, measurements, as well as methods of calculation and interpretation of results are summarized and discussed. It is revealed that researchers do not adhere to a single methodology for assessing the hemolytic activity of in-vitro polymeric materials. The differences are in the use of human blood or different animals, the ratio of the sample size of the test material to the volume of blood or erythrocyte suspension, the concentration of erythrocytes in the samples, the media for dilution of blood or erythrocyte mass during sample preparation, incubation time, time and relative acceleration or centrifuge revolutions during sample centrifugation.

Komin A.V., Leshukova N.S. In-vitro evaluation of the hemolytic effect of polymeric materials and products for use in medicine. Technologies of Living Systems. 2024. V. 21. № 4. Р. 80-92. DOI: https://doi.org/10.18127/j20700997-202404-09 (In Russian).

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