Всеволод Вячеславович Павшинцев - аспирант, биологический факультет, МГУ им. М.В. Ломоносова. E-mail: vsevolodpav@mail.ru Никита Александрович Митькин - аспирант, Институт молекулярной биологии им. В.А. Энгельгардта РАН (Москва). E-mail: mitkin.n.a@gmail.com Екатерина Александровна Кушнир - к.б.н., науч. сотрудник, ООО «НИИ Митоинженерии МГУ» (Москва). E-mail: ekaterina.a.kushnir@gmail.com Ольга Александровна Аверина - к.б.н., науч. сотрудник, ООО «НИИ Митоинженерии МГУ» (Москва). E-mail: averi-na.olga.msu@gmail.com Максим Львович Ловать - к.б.н., науч. сотрудник, биологический факультет, МГУ им. М.В. Ломоносова. E-mail: lovat@mail.ru

Изучено влияние активной иммунизации к ферменту метаболизма этанола - алкогольдегидрогеназе (с целью снижения активности данного фермента) на потребление 15 %-ного раствора этанола алкоголизированными крысами Wistar. В результате введения препарата животные демонстрировали значимое снижение потребление раствора этанола при свободном выборе с водой, при этом значимых изменений в поведенческих и биохимических тестах не наблюдалось.

 

1. Crow K.E., Hardman M.J. Regulation of rates of ethanol metabolism // Human metabolism of alcohol. 1989. V. 2. P. 3-16.
2. Plapp B.V., Leidal K.G., Murch B.P., Green D.W. Contribution of liver alcohol dehydrogenase to metabolism of alcohols in rats // Chemico-bio­logical interactions. 2015. V. 234. P. 85-95.
3. Galter D., Carmine A., Buervenich S., Duester G., Olson L. Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain // European Journal of Bio­chemistry. 2003. V. 270. № 6. P. 1316-1326.
4. Haseba T., Duester G., Shimizu A., Yamamoto I., Kameyama K., Ohno Y. In vivo contribution of Class III alcohol dehydrogenase (ADH3) to alco­hol metabolism through activation by cytoplas­mic solution hydrophobicity // Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2006. V. 1762. № 3. P. 276-283.
5. Kedishvili N., Gough W.H., Davis W., Parsons S, Li T.K., Bosron W.F. Effect of cellular retinol-binding protein on retinol oxidation by human class IV retinol/alcohol dehydrogenase and inhi­bition by ethanol // Biochemical and biophy­sical research communications. 1998. V. 249.  № 1. P. 191-196.
6. Mardh G., Dingley A.L., Auld D.S., Vallee B.L. Human class II (pi) alcohol dehydrogenase has a redox-specific function in norepinephrine meta­bolism // Proceedings of the National Academy of Sciences. 1986. V. 83. № 23. P. 8908-8912.
7. Meiser J., Weindl D., Hiller K. Complexity of dopamine metabolism // Cell Commun. Signal. 2013. V. 11. № 1. P. 34.
8. McCaffery P., Drager U.C. High levels of a reti­noic acid-generating dehydrogenase in the meso-telencephalic dopamine system // Proceedings of the National Academy of Sciences. 1994. V. 91.  № 16. P. 7772-7776.
9. Reuster T., Buechler J., Winiecki P., Oehler J. Influence of reboxetine on salivary MHPG con­centration and cognitive symptoms among pati­ents with alcohol-related Korsakoff\'s syndrome // Neuropsychopharmacology: official publica­tion of the American College of Neuropsyc­hopharmacology. 2003. V. 28. P. 974-978.
10. Eisenhofer G., Kopin I.J., Goldstein D.S. Catecho­lamine metabolism: a contemporary view with im­plications for physiology and medicine // Phar­macological reviews. 2004. V. 56. P. 331-349.
11. Svensson S., Some M., Lundsjö A., Helander A., Cronholm T., Höög J.O. Activities of human al­cohol dehydrogenases in the metabolic pathways of ethanol and serotonin // Eur. J. Biochem. 1999. V. 262. № 2. P. 324-329.
12. Potter J.J., Rennie-Tankersley L., Mezey E. Endotoxin enhances liver alcohol dehydrogenase by action through upstream stimulatory factor but not by nuclear factor-κB // Journal of Biological Chemistry. 2003. V. 278. № 6. P. 4353-4357.
13. Crabb D.W., Matsumoto M., Chang D., You M. Overview of the role of alcohol dehydrogenase and aldehyde dehydrogenase and their variants in the genesis of alcohol-related pathology // Proceedings of the Nutrition Society. 2004.  V. 63. № 1. P. 49-63.
14. Raskin N.H., Sokoloff L. Ethanol-induced adap­tation of alcohol dehydrogenase activity in rat brain // Nature.1972. V. 236. № 66.  P. 138-140.
15. Kravos M., Malešič I., Levanič S. Serum alcohol dehydrogenase levels in patients with mental disorders // Clinicachimicaacta. 2005. V. 361. № 1. P. 86-94.
16. Meier-Tackmann D., Agarwal D.P., Goedde H.W. Plasma alcohol dehydrogenase in normal and alcoholic individuals // Alcohol and Alcoholism. 1984. V. 19. № 1. P. 7-12.
17. Cappellano G., Woldetsadik A.D., Orilieri E., Shiva­kumar Y., Rizzi M., Carniato F., Gigliotti C.L., Boggio E., Clemente N., Comi C., Dianzani C., Boldorini R., Chiocchetti A., Renò F., Dianzani U. Subcutaneous inverse vaccination with PLGA particles loaded with a MOG peptide and IL-10 decreases the severity of experimental autoim­mune encephalomyelitis // Vaccine. 2014. V. 32. № 43. P. 5681-5689.
18. Ashmarin I.P., Danilova R.A., Obukhova M.F., Rud-ko O.I., Andreeva L.A. Long-lasting Changes of Albino Rats Behavior and Brain Bioamines Content After Immunization Against Cholecysto­kinin-3 and-4 // Neurochemical research. 2007. V. 32. № 3. P. 395-399.
19. Ashmarin I.P., Danilova R.A., Rud\'ko O.I., Belopol­skaya M.V., Obukhova M.F., Shmalgausen E.V. An immunochemical approach to model and long-term suppression of depressive and anxiety behavior // The Spanish journal of psychology. 2006. V. 9. № 2. P. 219-227.
20. Assier E., Semerano L., Duvallet E., Delavallée L., Bernier E., Laborie M., Boissier M.C. Modulation of anti-tumor necrosis factor alpha (TNF-α) antibody secretion in mice immunized with TNF-αkinoid // Clinical and Vaccine Immunology. 2012. V. 19. № 5. P. 699-703.
21. Jia T., Pan Y., Li J., Wang L. Strategies for active TNF-vaccination in rheumatoid arthritis treatment // Vaccine. 2013. V. 31. № 38. P. 4063-4068.
22. Durez P., Vandepapeliere P., Miranda P., Toncheva A., Berman A., Kehler T., Mociran E., Fautrel B., Mariette X., Dhellin O., Fanget B., Ouary S., Grouard-Vogel G., Boissier M.C. Therapeutic vaccination with TNF-kinoid in TNF antagonist-resistant rheumatoid arthritis: a phase II randomized, controlled clinical trial // PloSone. 2014. V. 9. № 12. e113465.
23. Kovalenko O.A., Ovcharyk E.M., Bondarenko O.V., Makarchuk M.Yu. Influence of behavioral reactions on ability to training of rats with different degree of alcoholic motivation // Visnyk of Luhansk National Taras Shevchenko University: Medical sciences. 2010. V. 21.  № 208. P. 54-59.
24. Estonius M., Hjelmqvist L., Jörnvall H. Diversity of vertebrate class I alcohol dehydrogenase. Mammalian and non-mammalian enzyme functions correlated through the structure of a ratite enzyme // Eur. J. Biochem. 1994. V. 224. № 2. P. 373-378.
25. Zubova O.B., Batalova I.P., Oknina N.B., Ko- gan R.D., Govallo I.V. Effect of neuroimmuni­zation with a complex of antigens from different portions of the brain on behavior in the rat // FiziolZh SSSR im. I.M. Sechenova. 1985. V. 71.  № 12. P. 1553-9.
26. Yoshimoto K., McBride W.J., Lumeng L., Li T.K. Alcohol stimulates the release of dopamine and serotonin in the nucleus accumbens // Alcohol. 1992. V. 9. № 1. P. 17-22.
27. Filatova E.V., Egorov A.Iu., Kucher E.O., Kulagi-na K.O. Effect of individual peculiarities on formation of preference of ethanol in female and male Wistar rats // Zh. Evol. Biokhim. Fiziol. 2011. V. 47. № 5. P. 404-410.
28. Shabanov P.D., Roik R.O., Lebedev A.A., Strel\'t-sov V.F. Effects of antidepressants in models of brain self-stimulation and place preference after prolong social isolation and alcoholization // Eksp. Klin. Farmakol. 2006. V. 69. № 4. P. 60-65.
29. Titkova A.M., Epstein O.I. Effect of preparations from potentiated ethanol on the content of biogenic monoamines and metabolism of ethanol in tissues of rats during alcoholization // Bull. Exp. Biol. Med. 2003 Jan. V.135. Suppl 7. P. 36-38.
30. El-Rahman R.S.A., Suddek G.M., Gameil N.M., El-kashef H.A. Protective potential of MMR vaccine against complete Freund-s adjuvant-induced in­flammation in rats // Inflammopharmacology. 2011. V. 19. № 6. P. 343-348.
31. Esteve E., Ricart W., Fernandez-Real J.M. Dys­lipidemia and inflammation: an evolutionary conserved mechanism // Clinical Nutrition. 2005. V. 24. № 1. P. 16-31.