V.V. Pavshintsev - Post-graduate Student, Faculty of Biology, M.V. Lomonosov Moscow State University. E-mail: vsevolodpav@mail.ru N.A. Mitkin - Post-graduate Student, Engelhardt Institute of Molecular Biology of Russian Academy of Sciences, Moscow. E-mail: mitkin.n.a@gmail.com E.A. Kushnir - Ph.D. (Biol.), Research Scientist, Mitoengineering Inst., MSU, Moscow. E-mail: ekateri-na.a.kushnir@gmail.com O.A. Averina - Ph.D. (Biol.), Research Scientist, Mitoengineering Inst., MSU, Moscow. E-mail: averi-na.olga.msu@gmail.com M.L. Lovat - Ph.D. (Biol.), Research Scientist, Faculty of Biology, M.V. Lomonosov Moscow State University. E-mail: lovat@mail.ru

Alcohol addiction treatment is one of the main unsolved problems of modern narcology. Enzymes of alcohol dehydrogenase (ADH) family - the main enzymes of ethanol metabolism - are also known to promote oxidation of some types of neurotransmitters, hormones and their catabolites. ADH oxidizes substrates containing hydroxyl groups, but the reverse reaction is also possible with lower efficiency. ADH is known to metabolize steroid hormones, GABA and catecholamines with high affinity. Deficiency of neurotransmitters (dopamine and serotonin) associated with chronic alcohol consumption is responsible for persistent depressive condition and formation of pathological ethanol craving. In this study, we applied active immunization against alcohol dehydrogenase to reduce developing alcohol motivation in Wistar rats. We demonstrated that this approach led to normalization of range of behavioral and biochemical parameters in alcohol-addicted rats. Immunization caused significant 10 days long (till the euthanasia) reduction of 15% ethanol consumption by animals. Biochemical blood analysis did not reveal any toxic effect of immunization.

 

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