O.V. Stepanova1, A.I. Kuznetsov2, D.O. Lyalin3, M.I. Lukyanov4, O.S. Stepanova5, G.A. Konoplev6

1, 3–6 Department of Photonics, St. Petersburg Electrotechnical University “LETI” (St. Petersburg, Russia)
2 Ldiamon AS (Tartu, Republic of Estonia)
1ovstepanova@stud.eltech.ru; 3dolyalin@stud.eltech.ru; 4milukyanov@stud.etu.ru; 5osstepanova@etu.ru; 6gakonoplev@etu.ru

Nutrition is an important and often underestimated factor influencing the progression of malignant tumors. It has been shown earlier that free dietary nucleotides present in fish and meat products can potentially accelerate tumor growth. Therefore, assessing the nucleotide content in ready-to-eat dishes prepared from fish, meat and seafood may be useful for developing more effective treatment strategies. The aim of this study is to evaluate the feasibility of using ATP bioluminescence and Fast Protein and Metabolite Liquid Chromatography (FPMLC) methods to analyze relative changes in the levels of free ATP and other purine nucleotides — ADP, AMP, IMP, inosine, and hypoxanthine — in the muscle tissue of meat and fish during cold storage in raw form and after thermal processing. It was revealed that conventional culinary treatment and high-pressure homogenization could dramatically change free nucleotide content, especially non-stable ATP, in ready-to-eat fish and meat products. This study demonstrated the capability of using ATP bioluminescence and FPMLC as rapid analytical methods for investigating the nucleotide composition of thermally processed meat and fish products, as well as for assessing potential risks associated with their consumption by patients with malignant tumors.

Stepanova O. V., Kuznetsov A.I., Lyalin D.O., Lukyanov M.I., Stepanova O.S., Konoplev G.A. Rapid assessment of potentially oncogenic dietary nucleotides by bioluminescence and chromatographic methods. Biomedicine Radioengineering. 2025. V. 28. № 5.
P. 31–34. DOI: https:// doi.org/10.18127/j15604136-202505-07 (In Russian)

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