K.T. Momynaliev1, I.A. Appolonova2, I.V. Ivanov3

1,3 Federal State Budget Institution «Russian Scientific and Research Institute for Medical Engineering»
of Federal Service for Supervision in the sphere of public health (Moscow, Russia)

2 Moscow Bauman State Technical University (Moscow, Russia)

1 kmomynaliev@vniiimt, 2 apollonova-i@bmstu.ru, 3 ivanov@vniiimt.ru

The ability to rapidly detect and distinguish between multiple infectious organisms in humans and the environment is critical to controlling the spread of infections. In addition, at present, it is important to identify infectious agents of bioterrorism in the field, that is, when work to identify infectious agents is associated with unsettled work and life, and these works are carried out outside settlements.

The aim of our review is formulation of requirements for molecular systems for use in the field to detect infectious agents and review existing solutions for rapid pathogen diagnosis in the field.

The main automatic systems for molecular diagnostics of pathogens, which satisfy the conditions of automation and multiplexity and which are registered as medical devices, are considered. Of the molecular platforms we have considered, the approach implemented in the FilmArray system allows multiplex testing of pathogens, but is easy to use, requires minimal time to set up the analysis, and is portable. Essentially, FilmArray is an implementation of the Lab on a Chip, or μTAS (Total Analysis Micro System).

It was revealed that microfluidic PCR should become one of the main directions of PCR technology for field research. Taking advantage of microfluidics, fast PCR can be performed in a portable device with great convenience. Considering compatibility with the most commonly used nucleic acid extraction methods and a simple heating strategy, microfluidic platforms can be considered as one of the competitive rapid PCR platforms in field testing due to its ultra-high thermal cycling efficiency with a sufficiently large reactor and automatic isolation of NAs from biosamples.

Momynaliev K.T., Appolonova I.A., Ivanov I.V. Approaches to the design of molecular platforms for use in the field to detect infectious agents. Biomedicine Radioengineering. 2023. V. 26. № 5. Р. 17-32. DOI: https://doi.org/10.18127/j15604136-202305-03 (In Russian).

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