Article or book title, author's surname, DOI

IR spectroscopy in molecular diagnostics of the maize seeds of pure lines and hybrids

Г.В. Максимов1, Е.В. Тютяев2, И.В. Сюсин3, Ч.Н. Раденович4

research article Pages: 60-62

  1. 1 д.б.н., профессор, кафедра биофизики, биологический факультет, МГУ им. М.В. Ломоносова
  2. 2 аспирант, кафедра биохимии, мл. науч. сотрудник, НОЦ «Нанобиотехнологии» Национально-исследовательского Мордовского государственного университета им. Н.П. Огарёва
  3. 3 аспирант, кафедра биотехнологии, мл. науч. сотрудник, НОЦ «Нанобиотехнологии» Национально-исследовательского Мордовского государственного университета им. Н.П. Огарёва
  4. 4 науч. советник, профессор, Университ в г. Ниш (Сербия).; www.cradenovic.com

Abstract

Using IR spectroscopy revealed that the maximum change in the spectra of components and clean hybrids of maize lines exposed C-H bonds of the molecules of unsaturated and saturated hydrocarbons , and C = O amide 1 protein and vibrations of OH bonds alcohols, indicating the different conformations of the molecules , causing the stability and plant physiological activity. It was found that the structure of the IR spectra of maize hybrids , similar to the structure of the IR spectra of pure lines: most pronounced oscillations of valence C-H bonds of alkenes and alkenes (2852 cm-1, 2926 cm-1), ( 995 cm-1) and the O-H bond (1161 cm-1 and 1082 cm-1) of the secondary and tertiary alcohols and C = O amide communications proteins 1 and 2 ( 1651 cm-1, 1541 cm-1) and aldehydes, ketones and α- amino acids (1747 cm-1). The maximum intensity of the bands of the IR spectrum is observed as oscillations in the valence bonds of alkenes (995 cm-1) seed varieties ZP434, and bands of the IR spectrum in 1161 cm-1 and 1082 cm-1 characterizing the deformation vibrations of OH bonds in alcohols and 1651 cm-1 (C = O stretching band communication amide 1 protein). We have shown that severe deformation ( 1460 cm-1) and the symmetric and asymmetric stretching vibrations of molecular C-H bonds ( 2852 cm-1, 2926 cm-1) are more common varieties ZP 505 and less than 341-ZP.

How to cite

Максимов Г.В., Тютяев Е.В., Сюсин И.В., Раденович Ч.Н. Метод инфракрасной спектроскопии в молекулярной диагностике состояния семян чистых линий и гибридов кукурузы // Технологии живых систем. 2014. № 2. С. 60–62.

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