Biotechnological aspects of enzymesand immunoglobulinsimmobilization on niosomes

L.V. Lyapustina; I.V. Zharnikova; T.V. Zharnikova; M.E. Mikhailova; D.A. Kovalev; E.V. Zhdanova

Biotechnological aspects of enzymesand immunoglobulinsimmobilization on niosomes

L.V. Lyapustina¹, I.V. Zharnikova², T.V. Zharnikova³, M.E. Mikhailova⁴, D.A. Kovalev⁵, E.V. Zhdanova⁶

research article Pages: 54-59

¹ Head of Laboratory Training of FGHI Stavropol Antiplague Institute of Rospotrebnadsor
² Dr. Sc. (Boil.), Leading Research Scientist, FGHI Stavropol Antiplague Institute of Rospotrebnadsor
³ Senior Research Scientist, FGHI Stavropol Antiplague Institute of Rospotrebnadsor
⁴ Research Scientist, FGHI Stavropol Antiplague Institute of Rospotrebnadsor
⁵ Leading Research Scientist, FGHI Stavropol Antiplague Institute of Rospotrebnadsor
⁶ Ph.D. (Biol.), Senior Research Scientist, FGHI Stavropol Antiplague Institute of Rospotrebnadsor

Keywords: enzyme immunoassay immobilized enzymes immuno¬peroxidase conjugate liposomes niosomes horseradish peroxidase

Abstract

The purpose of these studies  antibodies and enzyme immobilization by incorporating them in niosomalnye vesicles containing nonionic detergent, dicetyl phosphate and cholesterol. The optimal ratio of the components are found, methods of immobilization to ensure the effectiveness of the inclusion of the enzyme and immunoglobulins in niosomes are defined. On the basis of niosomesa highly stable and immunoperoxidase preparation for making an enzyme immunoassay to detect pathogens of tularemia and brucellosis are obtained.

How to cite

Ляпустина Л.В., Жарникова И.В., Жарникова Т.В., Михайлова М.Е., Ковалёв Д.А., Жданова Е.В. Биотехнологические аспекты иммобилизации ферментов и иммуноглобулинов на ниосомах // Технологии живых систем. 2014. № 2. С. 54–59.

References

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http://www.biorosinfo.ru/Vcongress/Krasilnykov.pdf
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[1] Barsukov L.I. Liposomy' membran // Sorosovskij obrazovatel'ny'j zhurnal. 1998. № 10. S. 2-9.

[2] Patent № 2320323 (RF). Sistema dostavki biologicheski aktivny'x veshhestv s pomoshh'yu niosom / I.A. Bazikov, P.A. Omel'yanchuk.

[3] Egorova T.A., Klunova S.M., Zhivuxina E.A. Osnovy' biotexnologii: Ucheb. posobie dlya vy'ssh. ped. ucheb. zavedenij, 3-e izd., ster. M.: Izd. czentr «Akademiya». 2006. S. 28.

[4] Erdakova V.P. Teoreticheskie i prakticheskie osnovy' konstruirovaniya sovremenny'x kosmeczevticheskix sredstv, obladayushhix transdermal'noj aktivnost'yu: monografiya. Bijsk: Izd-vo Alt. gos. texn. un-ta. 2008. S. 326.

[5] Sazy'kin Ju.O., Orexov S.N., Chakaleva I.I. Biotexnologiya: Ucheb. posobie dlya stud. vy'ssh. ucheb. zavedenij / pod red. A.V. Katlinskogo. 2-e izd. M.: Izd. czentr «Akademiya». 2007. S. 30-38.

[6] Dhand R. New frontiers in aerosol delivery during mechanical ventilation // Respir Care. 2004. № 49. P. 666-677.

[7] Nakane P.K., Kawaoi A. Peroxidase-labelled antibody-a new method of conjugation // J. Histochem. Cytochem. 1974. V. 22. № 4. P. 506-508; 1084-1091.

[8] Clark M.F., Adams A.N. Characteristics of the microplate method of enzyme-linked immunesorbent assay for the detection of plant virus // J. Gen. Virol. 1977. V. 36. № 3. P. 475-483.

[9] http://www.biorosinfo.ru/Vcongress/Krasilnykov.pdf

[10] http://spravocnikpolekarstvam.ru/Niosomy'/mikrokapsulynanokapsulyniosomy. htm

Abstract

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