E.V. Proskurnina1, Z.V. Moskvina2, M.M. Sozarukova3, M.V. Fedorova4, D.A. Okhobotov5, A.A. Kamalov6

1 Research Centre for Medical Genetics (Moscow, Russia)

2 City Polyclinic № 46 of the Moscow Department of Health (Moscow, Russia)

3 Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences (Moscow, Russia)

4 Central Research Institute for Epidemiology (Moscow, Russia)

5,6 Lomonosov Moscow State University (Moscow, Russia)

1 proskurnina@gmail.com, 2 gricukzv@yandex.ru, 3 s_madinam@bk.ru, 4 theklazontag@yandex.ru, 5 14072003m@gmail.com, 6 armais.kamalov@rambler.ru

Here, we aimed to studying the diagnostic significance of the assessment of the oxidative activity of neutrophils in prostate secretion using a complex chemiluminometric protocol using two enhancers (luminol and lucigenin) and neutrophil stimuli (phorbol-12-myristate-13-acetate, PMA, and N-formylmethionyl-leucyl-phenylalanine, fMLP).

An observational, single-stage, uncontrolled, monocenter pilot study included 13 patients aged 29 to 70 years with a confirmed diagnosis of chronic prostatitis, including in combination with benign prostatic hyperplasia. The material for the study was the secretion of the prostate gland, obtained after a massage of the prostate. Neutrophil activity was determined according to a complex protocol including the use of two chemiluminescent probes (luminol or lucigenin) and two stimuli (PMA or fMLP). This protocol allows to evaluate different pathways and links of neutrophil activation. Since neutrophils are the main sources of reactive oxygen species in bacterial prostatitis, the antioxidant capacity of the biomaterial was additionally assessed to assess the severity of oxidative stress.

The main results of the work are as follows: 1) the oxidative activity of neutrophils depends on the severity of inflammation, and increases in the series remission < latent course < exacerbation; 2) the magnitude of neutrophil activity depends not only on the severity of inflammation, but also on the nature of the infecting agent; 3) a strong direct correlation is typical for indicators of spontaneous luminol- and lucigenin-dependent chemiluminescence, PMA-stimulated luminol- and lucigenin-dependent chemiluminescence, PMA- and fMLP-stimulated luminol-dependent chemiluminescence, which makes it possible to use one of the most sensitive indicators, that is PMA-stimulated luminol-dependent chemiluminescence.

Thus, the activity of neutrophils in the secretion of the prostate gland is an informative diagnostic parameter. To assess the severity of inflammation, it is advisable to use the protocol of PMA-stimulated luminol-dependent chemiluminescence, since this parameter is characterized by a significantly higher analytical sensitivity than spontaneous chemiluminescence, and the indicators of fMLP-induced chemiluminescence and lucigenin-activated chemiluminescence correlate with it. When evaluating the result, one should take into account not only the severity of inflammation, but also the nature of the infecting agent.

Proskurnina E.V., Moskvina Z.V., Sozarukova M.M., Fedorova M.V., Okhobotov D.A., Kamalov A.A. Oxidative activity of prostate secretion neutrophils in bacterial prostatitis. Technologies of Living Systems. 2024. V. 21. № 4. Р. 42–53. DOI: https://doi.org/10.18127/ j20700997-202404-05 (In Russian).

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