A.S. Geogjayan1, A.G. Koshkidko2, I.V. Zharnikova3, E.V. Zhdanova4, O.A. Gnusareva5, D.V. Rusanova6, A.M. Zhirov7, M.M. Kurnoskina8, O.V. Vasilieva9, T.V. Zharnikova10, A.D. Besedin11

1–11 FGHI Stavropol Antiplague Institute of Rospotrebnadzor (Stavropol, Russia)

2 koshkidko-94@mail.ru, 3 IVJ-biotech@yandex.ru

Tularemia is a natural focal zoonotic infection, the natural foci of which are widespread in most of the territory of the Russian Federation. The causative agent of tularemia Francisella tularensis has a high pathogenicity for humans, which determines the place of this pathogen in the highest category A, among the most dangerous pathogenic microorganisms of potential agents of biological weapons and bioterrorism. In this regard, methods of laboratory diagnosis of tularemia and indication of its causative agent are of great importance, allowing to find out the cause of epidemic complications in a timely manner and to carry out anti-epidemic measures.

Objective of the work – to evaluate the use of magnoimmunosorbents in enzyme immunoassay and lyophilized erythrocyte diagnosticum in indirect hemagglutination reaction for indication of tularemia pathogen in environmental objects.

Immunoenzyme analysis with magnoimmunosorption and indirect hemagglutination reaction have sufficient sensitivity, are easy to use in the indication of tularemia pathogen. Tests of magnoimmunosorbents application in immunoenzyme analysis and diagnostic erythrocyte set showed the possibility of tularemia pathogen indication in environmental objects with confirmed data in polymerase chain reaction.

The efficiency of application of the developed preparations in the detection of tularemia pathogen in environmental objects has been proved.

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