O.A. Nagaeva1, L.N. Shcherbakova2, D.B. Revina3, M.V. Alexeenkova4, N.I. Zaytseva5, K.I. Kirillova6, L.M. Samokhodskaya7, O.B. Panina8

1–5,8 Lomonosov Moscow State University (Moscow, Russia)

2,6,7 Medical scientific and educational center of Lomonosov Moscow State University (Moscow, Russia)

1 nagaeva.99@inbox.ru, 2 lll2906@gmail.com, 3 lozinskaya.daria@gmail.com, 4 m.alexeenkova@gmail.com, 5 tata-zaitseva@mail.com, 6 dkkirillova@gmail.com, 7 slm@fbm.msu.ru, 8 olgapanina@yandex.ru

Recurrent pregnancy loss (RPL) is one of the major challenges of modern obstetrics. The research of genetic polymorphisms involved in the pathogenesis of RPL is ongoing.

The aim of this work was to study the associations between the single nucleotide polymorphisms of genes, which are responsible for atherosclerosis, endothelial dysfunction, angiogenesis and RPL onset.

87 patients were included in the study. The main group consisted of 65 patients with a history of two or more consecutive pregnancy losses. The control group included 22 women with three or more deliveries at term and without episodes of pregnancy loss. Genotypes for 13 single-nucleotide polymorphisms (single-nucleotide polymorphism, SNP) were determined by real-time PCR: MTHFR 1298 A>C (rs1801131), MTHFR 677 C>T (rs1801133), F5 1691 G>A (rs6025), ESR1 -397 T>C (rs2234693), PGR -413 G>A (rs10895068), APOE 176 C>T (rs429358), NOS3 -786 T>C (rs2070744), NOS3 894 G>T (rs1799983), VEGFA -634 G>C (rs2010963), VEGFA 936 C>T (rs1109324), TNF -238 G>A (rs361525), IL1β -511 C>T (rs16944), IL6 -174 G>C (rs1800795).

The genotype GC -634 G>C of the VEGFA gene and the genotype GC -174 G>C of the IL6 gene are 2 times more common in patients with RPL, the differences between patients with RPL and the control group are significant. The carriage of the GC genotype -634 G>C VEGFA increases the risk of RPL by 4 times (OR 4.00 CI 95% (1.38-11.56)). The carriage of the GC genotype -174 G>C IL6 increases the risk of PNB by 3.11 times (OR 3.11  CI 95% (1.08-8.96)).

Determination of GC genotype -634 G>C VEGFA and GC genotype -174 G>C IL6 can be used to identify patients with a high risk of RPL.

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