A.F. Ishtulin1, N.V. Korotkova2, I.V. Matveeva3, S.L. Ishtulina4, I.V. Minaev5, P.M. Polyakova6

1–6 Ryazan State Medical University named after Academician I.P. Pavlov (Ryazan, Russia)

Object – to define the amount of homocysteine in the spermoplasm of patients with chronic prostatitis III B and varicocele of II and III grades accompanying with asthenozoospermia.

The object of the exploration was 90 men diagnosed with chronic prostatitis III B and varicocele of II and III grades accompanying with asthenozoospermia. The material for the investigation was spermoplasm derived by the centrifugation of sperm samples for 10 minutes at the speed of 1000 rpm. Chemical microscopic examination of the ejaculate was carried out on a sperm analyzer ("АФС -500-2", Russia) and light microscopy was used (microscope "MС 100 X", Austria). The amount of homocysteine was determined by competitive ELISA in spermoplasm on a microplate reader for enzyme immunoassay STAT FAX 2100 (USA). The principle of the establishment method is that there is the competitive reaction between homocysteine marked with biotin, unmarked homocysteine in the analyzed samples and in the control and sorbed antibodies that are specific to homocysteine. The intensity of coloring is conversly proportional to the concentration of homocysteine in the sample.

It was revealed that there was an increase in the amount of homocysteine in the spermoplasm among patients with chronic prostatitis III B and asthenozoospermia in 1.5 times. Among patients with varicocele of II and III grade and asthenozoospermia, the amount of homocysteine in the spermoplasm was increased in 1.7 times.

An increase of the amount of homocysteine in the sperm is related with a decrease in reproductive function among men, which can be considered as one of the possible markers for the diagnosis of male infertility.

Ishtulin A.F., Korotkova N.V., Matveeva I.V., Ishtulina S.L., Minaev I.V., Polyakova P.M. Homocysteine as the predictor of decreased reproductive function among men. Technologies of Living Systems. 2022. V. 19. № 3. Р. 55-62. DOI: https://doi.org/10.18127/ j20700997-202203-06 (In Russian)

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