M.E. Chalyi1, D.A. Okhobotov2, E.V. Afanasyevskaya3, A.A. Strigunov4,
E.K. Iakhiaev5, A.S. Tivtikian6, D.M. Kamalov7, V.K. Dzitiev8,
N.I. Sorokin9, A.A. Kamalov10

1–4, 6–10 Moscow Research and Education Center, Lomonosov Moscow State University (Moscow, Russia)

1–10 Lomonosov Moscow State University (Moscow, Russia)

One of the priority line of the health care system worldwide is the early detection of cancer, since cancer is the leading cause of death among the population. The best results of radical treatment and the rise of disease-free survival could be achieved by early cancer diagnosis, before its local spread and distant metastasis. By studying the characteristics of normal and tumor cells, it was found that malignant cells differ in electromagnetic properties. Namely, the absorption of electromagnetic energy of a certain frequency (about 400 MHz) by malignant tissues is much higher than that of normal tissues. This is due to the membrane permeability of cancer cells, which is disrupted, and it affects their intracellular ionic composition. Malignant cells have an increased concentration of sodium and chlorine ions, a lower concentration of calcium, potassium, zinc and magnesium, as well as a higher water content and another acidity profile. An external electric field causes the ions migration inside of the cells and makes them a dielectric material with ability to polarize. This polarization of the dielectric materials creates an internal electric field with converse effect to external field. The internal field reflects with such degree of polarization as an ability of that field to store the energy.

In 2004, an Italian physicist Clarbruno Vedruccio described the phenomenon of non-linear interaction, which can be useful for noninvasive detection of the electric properties difference in healthy and cancer tissue. He fixed that difference with Bioscanner® TRIMprob™ (Tissue Resonance InterferoMeter Probe). The probe produces an alternating electromagnetic field which stimulates a charged particles (molecules, ions, electrons and nucleuses) in a target tissue providing a secondary radiation (polarization). Exactly secondary radiation is different for healthy and cancer tissue. The Bioscanner TRIMprob inludes a detection probe, a spectrum receiver-analyzer and a monitor. The detection probe is a cylindrical tube with non-liner oscillator that generates waves. The oscillator emits electromagnetic waves at several frequencies, from 400 to 1350 MHz.  A spectrum receiver-analyzer records the signal intensity from the antenna, translating that into a mathematic model. The results of the interaction between the electromagnetic fields are visualized in graphics at the monitor. Diagnostics with a bioscanner is carried out when the probe contacts the area of ​​interest, i.e. without invasion into the human body. The results of pilot studies show the opportunity of using probe to test the neoplasms in breast, liver, colon, kidney, bladder and prostate. The methods sensitivity ranges from 84% to 98% according to different studies, specificity is about 42% - 90%, and accuracy ranges from 60% to 93%. Tissue resonance interferometer method seems to be promise for prostate cancer screening, we are holding research to analyze the reliability of this method. Tissue resonance interferometer method seems to be promise for prostate cancer screening, we are conducting research to analyze the reliability of this method.

Chalyi M.E., Okhobotov D.A., Afanasyevskaya E.V., Strigunov A.A., Iakhiaev E.K., Tivtikian A.S., Kamalov D.M., Dzitiev V.K., Sorokin N.I., Kamalov A.A. The role of tissue resonance interaction in cancer detection. Technologies of Living Systems. 2022. V. 19. № 1. Р. 5-13. DOI: https://doi.org/10.18127/j20700997-202201-01 (In Russian)

1. Chissov V.I., Starinsky V.V., Mamontov A.S., Danilova T.V. Algoritmy vyyavleniya onkologicheskikh zabolevanij u naseleniya Rossiyskoy Federatsii: Metodicheskie rekomendacii dlya organizatorov zdravookhraneniya, vrachej pervichnogo zvena, vrachej-specialistov. M.: 2009. (in Russian).
2. Bray F., Ferlay J., Soerjomataram I., Siegel R.L., Torre L.A., Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 2018. V. 68(6). P. 394–424.
3. van Ginneken V. Are there any Biomarkers of Aging? Biomarkers of the Brain. Biomedical Journal of Scientific & Technical Research. 2017. V. 1. 14 p.
4. Grassi L., Spiegel D., Riba M. Advancing psychosocial care in cancer patients. F1000Res. 2017. V. 6. P. 2083.
5. McPhail S., Johnson S., Greenberg D., Peake M., Rous B. Stage at diagnosis and early mortality from cancer in England. Br. J. Cancer. 2015. Mar 31. P. 112.
6. Cree I.A., Uttley L., Buckley Woods H., et al. The evidence base for circulating tumour DNA blood-based biomarkers for the early detection of cancer: a systematic mapping review. BMC Cancer. 2017. V. 17(1). P. 697.
7. Cancer control: early detection. WHO Guide for effective programmes. Geneva: World Health Organization; 2007 (http://apps.who.int/iris/bitstream/10665/43743/1/9241547338_eng. pdf, accessed 1 October 2016)
8. Ulz P., Perakis S., Zhou Q., et al. Inference of transcription factor binding from cell-free DNA enables tumor subtype prediction and early detection. Nat. Commun. 2019. V. 10(1). P. 4666.
9. Cree I.A., Uttley L., Buckley Woods H., et al. The evidence base for circulating tumour DNA blood-based biomarkers for the early detection of cancer: a systematic mapping review. BMC Cancer. 2017. V. 17(1). P. 697.
10. Heitzer E., Perakis S., Geigl J.B., Speicher M.R. The potential of liquid biopsies for the early detection of cancer. NPJ Precis Oncol. 2017. V. 1(1). P. 36.
11. Fricke H., Morse S. The electric capacity of tumors of the breast. Journal of Cancer Research. 1926. V. 10. P. 340–376.
12. Joines W.T. Frequency-dependent absorption of electromagnetic energy in biological tissue. IEEE Transactions on Biomedical Engineering. 1984. V. 31. № 1. P. 17–20.
13. Al Ahmad M., Al Natour Z., Mustafa F., Rizvi T.A. Electrical Characterization of Normal and Cancer Cells. IEEE Access. 2018.
    V. 6. P. 25979–25986.
14. Nelson D.L., Cox M.M., Lehninger M. Principles of Biochemistry. Fifth Ed. New York: Freeman WH. 2008. P. 1239–1254.
15. Grimnes S., Martinsen G. Bioimpedence and bioelectricity basics. Academic press. San Diego. 2000. P. 313–319.
16. Heileman K., Daoud J., Tabrizian M. Dielectric spectroscopy as a viable biosensing tool for cell and tissue characterization and analysis. Biosens. Bioelectron. 2013. Nov 15. V. 49. P. 348–359.
17. Cameron I., Smith N.K., Pool T.B., Sparks R.L. Intracellular concentration of sodium and other elements as related to mitogenesis and oncogenesis in vivo. Cancer Res. 1980. V. 40. № 5. P. 1493–1500.
18. Tejedor L., Modet S.G., Manchev L., Parikesit A.A. Breast Cancer and Breast Reconstruction. London, United Kingdom, Published. 2020. 184 p.
19. Spugnini E.P., Sonveaux P., Stock C., Perez-Sayans M., De Milito A., Avnet S., Garcìa A.G., Harguindey S., Fais S. Proton channels and exchangers in cancer. Biochim. Biophys. Acta. 2015. V. 1848(10 Pt B). P. 2715–2726.
20. Vedruccio C., Meessen A. EM Cancer Detection by Means of Non Linear Resonance Interaction, Proceedings of PIERS 2004, Progress in Electromagnetics Research Simposium, Pisa, Italy, March 28-31 2004. P. 909–912.
21. Balma M., Lacquaniti S., Fasolis G., Autino E. The potential of TRIMprob in the diagnosis of prostatic neoplasma: method of use and results of a study of healthy subjects. 2015. 10.13140/RG.2.1.2330.0964
22. Tubaro A., et al. The Electromagnetic Detection of Prostatic Cancer: Evaluation of Diagnostic Accuracy. Urology. 2008. V. 72. Iss. 2.
    P. 340–344.
23. Bellorofonte C., Vedruccio C., Tombolini P., Ruoppolo M., Tubaro A. Non-Invasive Detection of Prostate Cancer by Electromagnetic Interaction. European Urology. 2004. V. 47. Iss. 1. P. 29–37.
24. Gokce O., Sanli O.M., Salmaslioglu A., Tunaci A., Ozsoy C., Ozcan F. Tissue Resonance Interaction Method (TRIMprob) has the potential to be used alongside the recognized tests in the screening protocols for prostate cancer. International journal of urology: official journal of the Japanese Urological Association. 2009. V. 16(6). P. 580–583.
25. Da Pozzo L., Scattoni V., Mazzoccoli B., Rigatti P., Manferrari F., Martorana G., Pietropaolo F., Belgrano E., Prezioso D., Lotti T., Villari D., Nicita G. Tissue‐resonance interaction method for the noninvasive diagnosis of prostate cancer: analysis of a multicentre clinical evaluation. BJU International. 2007. V. 100. P. 1055–1059.
26. Di Viccaro D., Perugia G., Cerulli C., Olivieri V., Bova G., Balla J., Zanza C., Teodonio S., Liberti M. The Accuracy of Tissue Resonance Interaction Method Probe (Trimprob Tm) in Non-Invasive Diagnosis of Prostatic Cancer. Analysis of the Results of 782 Patients. Urologia Journal. 2009. V. 76. № 4. Suppl. 15. P. 1–3.
27. Guimaraes G.C., Costa W.H., Rosa R.A., Zequi S., Favaretto R. Predictive role of Trimprob associated with multiparametric MRI in the diagnosis of prostate cancer. International Braz. J. Urol.: official journal of the Brazilian Society of Urology. 2017. V. 43(1).
    P. 29–35.