V.V. Egorov – 

Dr. Sc. (Chem.), Professor, Chemistry department, Moscow State Academy of Veterinary  Medicine and Biotechnology n.a. K. I. Scriabin 

E-mail: kaf_chimii@mgavm.ru

O.A. Lopatina – 

Ph.D. (Med.), Leading Research Scientist, Tissue Culture Laboratory, FSBI “National Center  

of Epidemiology and Microbiology named after honorary academician N.F.Gamaleya” of the Ministry of Health  of the Russian Federation (Moscow)

E-mail: lopatina.online@yandex.ru

O.V. Baklanova – 

Ph.D. (Med.), Head Research Scientist, Tissue Culture Laboratory, FSBI “National Center  of Epidemiology and Microbiology named after honorary academician N.F.Gamaleya” of the Ministry of Health of the Russian Federation (Moscow) 

E-mail: cells@rambler.ru 

I.A. Suetina – 

Ph.D. (Med.), Leading Research Scientist, Tissue Culture Laboratory, FSBI “National Center  of Epidemiology and Microbiology named after honorary academician N.F.Gamaleya” of the Ministry of Health of the Russian Federation (Moscow) E-mail: cells@rambler.ru 

M.V. Mezentseva – 

D.Sc. (Biol.), Head of Tissue Culture Laboratory, FSBI “National Center of Epidemiology and Microbiology named after honorary academician N.F.Gamaleya” of the Ministry of Health of the Russian Federation (Moscow) E-mail: marmez@mail.ru

supposed to be perspective in antitumor and antiviral treatment.

The effect of HPA on human cells remains an open question.

Influence of polyoxometalate (POM) aqua solutions with anionic surface-active substances (SAS) on proliferative activity of human cells was investigated.

Cytotoxic effect of HPAs reduce their application in drug development. It is well known, that HPA toxity can change. Encapsulation in micelles or vesicules of surface-active substances (SAS) can change HPA toxity.

In this work, the cytotoxic effect of polyoxometalate (POM) aqua solutions with anionic surface-active substances (SAS) on proliferative activity of human cells was learned.

POM Keggin (H3PW12O4) was taken. Surface-active substances was presented by oleic acid (OA) and dodecyl semiether of itaconyc acid (IA), kalium salt. The research was conducted on cells culture of human embrio fibroblast (HEF) and oncological bone marrow cells from patient with leukaemia (L-41). Growth-regulating cell activity was estimate by their viability and proliferative index. Cellulotoxic effect was studied by MTT-method on human embrio fibroblast (HEF) cells.

The study showed POM’s toxity on human embrio fibroblast (HEF) cells. Oleic acid did not reduce the toxity of POM on human embrio fibroblast (HEF) cells. MTT test showed that the lowest nontoxical dilution of HPA was 1/128. HPA + itaconyc acid combination demonstrated the elevation of cytotoxity on human embryo fibroblasts (HEF). The lowest nontoxical dilution of this combination was 1/1024. The study showed that HPA+oleic acid combination had less toxity effect. The lowest nontoxical dilution for HPA + oleic acis combination was 1/64.

POM and POM + surface-active substances (SAS) combination reduced growth activity of L-41 cells. Authors presuppose that there are different influence mechanisms of oleic acid and itaconyc acid on POM’s cytotoxity. 

1. Yanagie H., Ogata A., Mitsui S. et al. Anticancer activity of polyoxomolybdate // Biomed. Pharmacother. 2006. V. 60. P. 349–352. 
2. Rhule J.T., Hill C.L., Judd D.A. et al. Polyoxometalates in medicine // Chem. Rev. 1998.V. 98. P. 327–358.
3. Pope M.T. Heteropoly and Isopoly Oxometalates. Berlin: Springer Verlag. 1983.
4. Kovalevskij S.A., Lopatina O.A., Dalidchik F.I. i dr. Vozdejstvie geteropolikislot Keggina na kletki fibroblastov embriona cheloveka // Rossijskie nanotekhnologii. 2018. T. 13. № 7–8. S. 51. 
5. D'yakonov L.D. ZHivaya kletka v kul'ture (metody i primeneniya v biologii). M.: Kompaniya Sputnik. 2009. S. 231–2456.
6. Habriev R.U. Rukovodstvo po eksperimental'nomu (doklinicheskomu) izucheniyu novyh farmakologicheskih veshchestv. M.: Medicina. 2005. 832 s.
7. SHCHukin E.D., Percov A.V., Amelina E.A. Kolloidnaya himiya. M.: Izd-vo MGU. 1982. S. 229.
8. Egorov V.V. Suprun N.V. Klyamkin A.A. Vliyanie stroeniya PAV na ego sposobnost' k micello- i vezikuloobrazovaniyu // Vestnik MGU. Ser. 2. Himiya. 1996. T. 37. № 2. S. 178.
9. Egorov V.V. Radical Polymerization of Micelle-Forming Monomers in Water // J. Polymer Science. 1995. V. 33. P. 1727–1733.