R.R. Rakhmatullin, N.V. Perova, O.I. Burlutskaya, I.R. Gil-mutdinova, L.R. Adel-shina

Toxicity and biological compatibility of the bioplastic material «Hyamatrix» made by a method photoinducedcross of macromolecules in hydrogel on a basis nativ, chemically not modified hyaluronic acids in culturemesenchymalstromalnycages is investigated. Results cellular works and morphology studying testify to absence of significant changes in control and investigated group. The Morfometrichesky data on experiment specifies in some reduction of volumes of cages in investigated group 1884,51мкм3 (Min 152,63; Max 20059,53), against 3826,65 мкм3 (Min 350,93; Max 36733,7), at comparable speed proliferation that testifies to a good condition of cages at long cultivation. The estimation of ability of cages to taxis has shown similar results, the distance of movement of cages has made - 281,50мкм (Min 33,0; Max 722,0; ±154,78) in investigated group, against 234,00мкм (Min 23,0; Max 684,0; ±155,01) that testifies to absence of negative influence «Hyamatrix» on cages. Results immunofenotipirovaniyehaven't revealed what or distinctions in the groups, all cages after cultivation express a standard set stromalnyantigenes of CD 44, 73, 90, 105 and were negative on haemopoetic markers of CD 14, 34, 45, HLA-DR. Ability of cages adhesion and to grow on an investigated material, has been proved by presence of the painted cages at coloring by dye «Gimza», and their kernels at coloring by specific dye DAPI, active only in the presence of DNA live cages. Results of electronic microscopy have confirmed presence characteristic fibroblastosimilarcages on material surfaces, and also their germination during a large time. Generalization of the presented experimental results allows to draw a conclusion on absence of cytotoxicity of a bioplastic material «Giamatriks» on a basis hyaluronic acids. The research was carried out on the base of the content ? «The Ural: history, economics, culture» 11-06-81602 and the administration of Orenburg region.