A.E. Gerasimenko1

1 St. Petersburg Electrotechnical University “LETI” (St. Petersburg, Russia)
1 sasha_grsmnk@mail.ru

The development of neurosurgery faces a key limitation – the lack of high-precision methods of operative monitoring of the condition of cerebral tissues, which leads to risks of damage to functional areas or incomplete removal of pathological foci. This problem requires the development of fundamentally new biotechnical solutions capable of objectively characterizing the state of nervous tissue at the cellular and subcellular levels directly during surgical intervention.

The aim of the work is to development of a biotechnical system (BTS) for in vitro diagnosis of brain tissue, which will provide neurosurgeons with information about the condition of tissues during operations for resection of brain tumors.

The use of developed BTS will allow conducting primary experiments to test the method for determining the state of brain tissue in vitro and develop a system for intraoperative determination of tumor boundaries by analyzing the functional state of surrounding tissues, which minimizes the risks of incomplete resection or damage to healthy structures. It will reduce postoperative complications and improve the prognosis of survival by removing the tumor tissue as completely as possible while preserving critical areas of the brain. In addition, the system can be integrated into neural navigation systems, providing the surgeon with objective data in real time. A scientific substantiation of the possibility of using the impedance measurement method to diagnose the condition of brain tissues in vitro has been carried out. A BTS scheme has been developed for in vitro diagnosis of the state of brain tissues, based on this method. The components of the BTS are described and the choice of blocks and elements is explained.

Gerasimenko A.E. Development of a biotechnical system for diagnosis the condition of brain tissues in vitro. Biomedicine Radioengineering. 2026. V. 29. № 3. P. 71–75. DOI: https:// doi.org/10.18127/ j15604136-202603-12 (In Russian)

1. Romashchenko P.N., Majstrenko N.A., Krivolapov D.S., Vshivcev D.O. Radionavigacionnye i fotodinamicheskie metodiki intraoperacionnoj vizualizacii okoloshchitovidnyh zhelez (obzor literatury). Vestn. Hir. 2020. №3 (In Russian).
2. Gerasimenko A.E. Rezul'taty eksperimental'nyh issledovanij izmeneniya vo vremeni chastotnyh harakteristik impedansa opuholej golovnogo mozga. Biotekhnosfera. 2023. № 1 S. 30–34 (In Russian).
3. Kenerson H.L., Sullivan K.M., Labadie K.P., Pillarisetty V.G., Yeung R.S. Protocol for tissue slice cultures from human solid tumors to study therapeutic response. STAR Protocols. 2021. V. 2. № 2. Art. № 100574.
4. Krivtsun I.V., Pentegov I.V., Sydorets V.M.,Rymar S.V. A technique for experimental data processing at modeling the dispersion of the biological tissue impedance using the Fricke equivalent circuit. EіE. 2017. №5 (eng).
5. Goncharov V.D., Samochernyh K.A., Voinov N.E., Kukanov K.K., Gerasimenko A.E., Yashkardin R.V., Gorelikova M.A. Teoreticheskie osnovy ispol'zovaniya spektroskopii impedansa v medicine. Rossijskij nejrohirurgicheskij zhurnal im. prof. A.L. Polenova. 2023. № 15(4). S. 35–41 (In Russian).
6. Kukanov K.K., Zrelov A.A., Samochernyh K.A., Olyushin V.E., Potemkina E.G., Ulitin A.Yu. Sravnitel'nyj analiz stereotaksicheskogo i endoskopicheskogo metodov biopsii opuholej golovnogo mozga (obzor literatury). Rossijskij nejrohirurgicheskij zhurnal im. prof. A.L. Polenova. 2020. № 12(1). S. 64–70 (In Russian).