N.O. Sitkov1, Z.M. Yuldashev2

1,2 St. Petersburg Electrotechnical University ETU «LETI», (St. Petersburg, Russia)
1 sitkov93@yandex.ru

Quantitative rapid detection of protein disease biomarkers is an important task in modern personalized medicine, since changes in their concentration in biological fluids reflect the dynamics of the pathological process and can be used for early diagnosis, therapy monitoring, and timely detection of exacerbations. Despite progress in the development of individual biosensing elements, the design of instrumented solutions for point-of-care diagnostics often remains fragmented: the choice of the recognition element, surface modification, signal registration, and data processing are addressed separately. This determines the need for a unified concept for constructing biosensor systems focused on reproducibility, technical feasibility, and subsequent metrological validation.

Objective – to develop a concept for constructing biosensor systems based on peptide recognition elements for the rapid diagnosis of protein disease biomarkers, relying on the integrated development of informational, methodological, instrumental, software-algorithmic, and metrological support levels.

A scientific rationale was provided for an approach to biosensor system design in which the protein structure is considered as the primary object of biosensing, while peptide recognition elements are regarded as a technologically compatible tool for selective molecular recognition. A conceptual framework for constructing biosensor systems was formulated, including five interrelated support levels: informational, methodological, instrumental, software-algorithmic, and metrological. A generalized structural design matrix was developed that defines the key tasks, output artifacts, and quality criteria at each level. It was shown that the proposed approach ensures logical consistency between the properties of the target protein as the detection object, the choice of peptide recognizer, the architecture of the sensor interface, the means of signal registration, interpretation algorithms, and metrological procedures.

The concept proposed and described in this work provides a methodological basis for the creation of next-generation biosensor platforms enabling reproducible development of rapid diagnostic systems for protein disease biomarkers, as well as their further engineering refinement and metrological validation.

Sitkov N.O., Yuldashev Z.M. The concept of constructing biosensor systems based on peptide recognition elements for express diagnostics of protein disease biomarkers. Biomedicine Radioengineering. 2026. V. 29. № 3. P. 29–36. DOI: https:// doi.org/10.18127/ j15604136-202603-05 (In Russian)

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