D.G. Chebotarev1, A.A. Ryltsev2, I.I. Naumov3, R.R. Ibadov4

1–4 Don State Technical University (Rostov-on-Don, Russia)

1 chebotarv01@mail.ru; 2 artem12345609@mail.ru; 3 inaumov@donstu.ru; 4 ragim_ibadov@mail.ru

The development of precision systems for generating electromagnetic fields in the optical range is a pressing issue in modern biomedical instrumentation. Controlling the vital functions of biological systems using precisely dosed optical radiation finds application in pharmaceuticals, biotechnology, and medicine. Existing systems often lack the ability to flexibly and dynamically control the spectral power density (SPD) in real time, limiting their use for obtaining biological material with specified functional properties and for scientific research into the mechanisms of photobiological interactions.

Objective – to develop the architecture and prototype of an automated multispectral irradiation system with feedback based on microcontroller PWM control and circuit design for stabilizing the current of LED emitters, and to experimentally evaluate its biomedical effectiveness on model biological specimens (Raphanus sativus L., Eruca sativa Mill.).

A structural and electrical circuit diagram of a system based on microcontroller control and pulse-width modulation (PWM) of semiconductor emitters using current stabilizers and feedback circuits for current and spectral parameters is proposed.

A laboratory setup with independent channels for controlling radiation intensity in the 400–800 nm range (blue, green, red, and far-red) was implemented. The ability of the developed system to modulate the metabolic response of biological objects was experimentally confirmed: a 40% increase in active biomass was achieved with a spectral configuration dominated by the green range (495–600 nm). Nonlinear relationships between the duty cycle of the PWM signal in the red channel and the intensity of cellular metabolism were identified.

The developed device can serve as a basic module for the creation of industrial bioreactors with controlled irradiation, closed-loop life support systems, and can also be used in fundamental research into the mechanisms of photobiological interactions.

Chebotarev D.G., Ryltsev A.A., Naumov I.I., Ibadov R.R. Development and experimental testing of a hardware and software complex for multispectral impact on biological objects. Biomedicine Radioengineering. 2026. V. 29. № 2. P. 85–98. DOI: https:// doi.org/10.18127/ j15604136-202602-08 (In Russian)

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