A.N. Briko1, A.V. Kobelev2, A.N. Dmitriev3, A.N. Tikhomirov4, S.I. Shchukin5, K.V. Kotenko6, I.I. Eremin7

1-5 Moscow Bauman State Technical University (Moscow, Russia)
1,6,7 Petrovsky National Research Center of Surgery (Moscow, Russia)

1 briko@bmstu.ru

Human adipose tissue contains adipose stem cells that can differentiate into various types of connective tissue cells when growth factors appear. This effect is currently actively used in regenerative and cosmetic medicine. The process of isolating adipose stem cells involves extracting the stromal vascular fraction. An urgent task is to develop a mechanical method for isolating the stromal-vascular fraction, which is based on damage to the stromal-vascular matrix using mechanical action and removal of large adipocytes. The advantage of this method is the absence of the use of special expensive enzymatic solutions.

Development of a laboratory stand for automated mechanical production of SVF with controlled exposure for standard designs of medical devices used in practice, intended for mechanical processing of adipose tissue.

The design of the laboratory stand makes it possible to evaluate the viscosity of lipoaspirate in real time, making it possible to carry out studies to quantitatively substantiate the parameters of the procedure for mechanically obtaining the stromal-vascular fraction to obtain a product with the required characteristics, depending on the initial quality of the lipoaspirate.

The developed laboratory stand makes it possible to study the processes and parameters that must be achieved for effective mechanical separation of lipoaspirate.

Briko A.N., Kobelev A.V., Dmitriev A.N., Tikhomirov A.N., Shchukin S.I., Kotenko K.V., Eremin I.I. Laboratory stand for assessing the parameters of mechanical production of the stromal-vascular fraction. Biomedicine Radioengineering. 2023. V. 26. № 5. Р. 102-113. DOI: https://doi.org/10.18127/j15604136-202305-11 (In Russian).

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