A.A. Rumyantseva1, A.A. Buslaev2, J.R. Huseynov3, I.P. Semchuk4, A.E. Kosorukov5, N.P. Muravskaya6, A.V. Samorodov7, A.K. Volkov8

1–7 Bauman Moscow State Technical University (Moscow, Russia)

8 Scientific and Educational Medical-Technological Center
Bauman Moscow State Technical University (Moscow, Russia)

Buccal epithelial cells have been an attractive area for researchers recently due to both the morphology of the cells and their location. These cells are thin polygonal plates lining the mucous membrane, originate in the oral cavity and end in the rectum, lining the entire digestive system, so they are the boundary between the internal and external environment of a living organism and have dimensions: from 48 to 142 microns in diameter, area – from 2000 to 7500 microns, and the size of the nuclei of buccal epithelium cells is from 8 to 15 microns, the area is from 28 to 90 microns, which allows to use an optical light microscope with micro lenses from 10x to 40x. This type of cells can be studied by the following methods: cytologically, immunocytochemically and electrokinetically. It is the movement of the nuclei of buccal epithelium cells, as well as the movement parameters and the percentage of mobile nuclei from the total number of cells in the studied preparation, when exposed to an electromagnetic field, that is an informative parameter in assessing the functional state of the human body, that is, determining the availability of reserves of the main systems of the human body. It becomes possible to achieve such a movement during such a phenomenon as microelectrophoresis. Such a phenomenon becomes a source of electrokinetic phenomena at the boundary of the radel of two media – mobile and stationary. In this study, the mobile medium is the moving nucleus, or rather the membrane of the nucleus, and the stationary one is the cytoplasm of the cell. At the same time, there is currently no justification for the choice of parameters and modes of exposure, namely, parameters for the occurrence of the phenomenon of microelectrophoresis, in order to achieve maximum diagnostic informativeness. Thus, one of the important and urgent tasks of this research in the entire biotechnical system is the development of a microelectrophoresis module consisting of an impact unit represented by a rectangular pulse generator, an Arduino Leonardo hardware platform, a personal computer, a power source where it will be possible to set certain parameters of the impacting signal, as well as a microelectrophoresis camera, which is represented by modified Goryaev chamber (metal plates are hermetically fixed on the edges of the chamber to conduct electric current, playing the role of an electrode system) and an oscilloscope to monitor the state of the module being developed to study the electrokinetic properties of buccal epithelial cells to assess the functional state of the human body, which is what this work is devoted to. This article also describes the method of collecting a database of buccal epithelial cells, the selection and testing of the parameters of the acting electromagnetic field, namely the intensity, duration and shape of the acting signal.

Rumyantseva A.A., Buslaev A.A., Huseynov J.R., Semchuk I.P., Kosorukov A.E., Muravskaya N.P., Samorodov A.V., Volkov A.K. Development of a microelectrophoresis module for studying the electrokinetic properties of buccal epithelial cells. Biomedicine Radioengineering. 2022. V. 25. № 5. Р. 70-78. DOI: https://doi.org/10.18127/j15604136-202205-08 (In Russian)

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