L.A. Morozova, S.V. Savel’ev

The Institute of Radioengineering and Electronies of Russian Academy of Sciences (Fryazino, Russia)

The physical properties of aqueous solutions of pharmacological preparations largely determine their therapeutic effect. Recently, pharmacological preparations based on high and ultrahigh dilution of basic medicinal substances are finding increasing use. An example of such effective drugs is the drug "Anaferon" containing antibodies to interferon gamma in a proportion of 10-15 to lactose, the drug's filler. However, the mechanism of action of such preparations containing the base substance in such small proportions is not clear. In this regard, the problem emerges, according to which physicochemical criteria to evaluate the difference between aqueous solutions of high and ultrahigh dilutions of substances from pure water, and what properties are pharmacologically determining.

In this regard, the problem arises of determining such physical parameters of aqueous solutions, the variation of which can be fixed depending on the concentration of the base substance, and therefore be able to indicate the percentage of the base substance at high and ultrahigh dilutions. At present, the aqueous solutions of these problems is especially acute due to the huge number of pharmacological preparations offered on the market for medicinal substances and the large number of pharmacological companies that produce them. It is necessary to develop a non-invasive method for the rapid and fairly accurate determination of the base substance and its concentration in aqueous solutions of the pharmacological preparations used, which allows it to be used both in laboratory and in the field.

The problem solved in this article is to show the fundamental possibility of creating a non-invasive method for determining the base substance and its concentration in aqueous solutions based on measuring the absorption of aqueous solutions of substances at normal, high and ultrahigh dilutions.

Morozova L.A., Savel’ev S.V. Physical properties of water solutions at high dilutions of basic substances. Biomedicine Radioengineering. 2021. V. 24. № 2. Р. 39−46. DOI: https://doi.org/10.18127/j15604136-202102-05 (in Russian).

1. Barthel J., Buchner R., Munsterer M. Electrolyte data collection. Part. 2: Dielectric properties of water and aqueous electrolyte solutions DECHEMA. Chemistry Data Series. 1995. V. 12. Part 2.
2. Broadband Dielectric Spectroscopy / Eds. F. Kremer, A. Schonhals. Berlin: Springer-Verlag. 2003. 729 p.
3. Lileev A.S., Filimonova Z.A., Lyashchenko A.K. Dielectric permittivity and relaxation in aqueous solutions of alkali metal sulfates and nutrates in the temperature range 288-313 K. J. Mol. Liq. 2003. V. 103-104. P. 299-308.
4. Lyashchenko A.K., Khar’kin V.S., Lileev A.S. Complex permittivityof low-concentration aqueous solutions of acetone. Russ. J. Phys. Chem. A. 2000. V. 74. P. 529-534.
5. Lyashchenko A.K., Khar’kin V.S., Lileev A.S. Complex permittivityand relaxation in aqueous solutions of etal ethil ketone.Russ. J. Phys. Chem. A. 2001. V. 75. P. 195-201.
6. Epstein O.I., Pavlov I.F., Shtark M.B. Improvement of Memory by Means of Ultra-low Doses of Antibodies to S-100B Antigen. Evidencebased Complementary and Alternative Medicine. 2006. V. 3. № 4. P. 541-545.
7. Epstein O.I., Beregovoy N.A., Sorokina N.S. et. al. Membrane and synaptic effects of anti-S100 are prevented by the same antibodies in low concentrations. Front. Biosci. 2003. V. 8. P. A79-A84.
8. Epstein O.I., Zarapova T., Simonova O.G. et. al. Plasticity of neuronal responses induced by low concentration of exogenous ligands effecting cellular calcium stores. Front. Biosci. 2004. V. 9. P. 809-815.
9. Jonsson M., Linse S., Frohm B. et. al. Semenogelins 1 and 2 bind zinc and regulate the activity of prostate-specific antigen. Biochem. J. 2005. V. 15, № 378. Pt 2. P. 447-453.
10. Delbancut A., Baroullet M.P., Cambar J. Evidence and mechanistic approach of the protective effects of heavy metal high dilutions in Rodent and renal cell cultures. Ed. M. Bastide. Dordrecht. 1997. P. 71-83.
11. Barthel J., Buchner R., Munsterer M. Electrolyte Data Collection. Part 2: Dielectric Properties of Water and Aqueous Electrolyte Solutions. Chemistry Data Serirs. V. XII. Part 2. FrankfurtamMain, Dechma. 1985. 365 p.
12. Lyashchenko A.K., Karatayeva I.M., Dunyashev V.S. Svyaz radioyarkostnykh i dielektricheskikh svoystv vodnykh rastvorov soley v millimetrovoy oblasti spectra. Zhurnal fizicheskoy khimii. 2019. T. 93. S. 552-557 (in Russian).
13. Krivoruchko V.I. Priyemnyy radiometricheskiy modul 5-millimetrovogo diapazona dlin voln s maloshumyashim usilitelem na vkhode // Izvestiya vuzov. Radiofizika. 2003. T. 46. № 8-9. S. 782-786 (in Russian).
14. Sinitsyn N.I., Elkin V.A. Osobaya rol strukturizatsii vodosoderzhashchey sredy v sovremennykh biomeditsinskikh radioelektronnykh tekhnologiyakh i nanotekhnologiyakh budushchego. Biomeditsinskiye tekhnologii i radioelektronika. 2007. № 2-4. S. 31–43 (in Russian).
15. Betskiy O.V., Morozova L.A., Savelyev S.V. Millimetrovyye i teragertsovyye volny v rastvorakh farmakologicheskikh preparatov biologicheskogo proiskhozhdeniya. Biomeditsinskaya radioelektronika. 2017. № 4. S. 42– (in Russian). Biomedicinskaya radioelektronika / Biomedicine Radioengineering, V. 24, № 2, 2021, P. 39−46