350 rub
Journal Biomedical Radioelectronics №2 for 2021 г.
Article in number:
Biological effect of microwave electromagnetic radiation: methods of registration and protection
Type of article: scientific article
DOI: https://doi.org/10.18127/j15604136-202102-03
UDC: 577.346 613.693 621.391
Authors:

D.V. Guryev¹, N.M. Smetanina², S.M. Rodneva³, A.A. Tsishnatti4, D.V. Molodtsova5,  Yu.A. Fedotov6, A.S. Dmitriev7, A.A. Artamonov8

1,2,3,4,5,6 Russian State Research Center Burnasyan Federal Medical Biophysical Center  of Federal Medical Biological Agency (Moscow, Russia)

7 Institute of Radio Engineering and Electronics named V. А. Kotelnikov RAS (Moscow, Russia)

8 The Russian Federation State Research Center – Institute of Biomedical Problems of RAS (Moscow, Russia)

Abstract:

The development of modern electronics, communications, high-tech production and other technologies lead to a significant increase in the intensity of microwave electromagnetic radiation. This article discusses the problem of registration (including biological detection) and protection from microwave electromagnetic radiation.

To consider the method of integral biological and instrumental recording of the intensity of microwave electromagnetic radiation and to evaluate the method of protection using a passive multifrequency re-emitter generator with a radio holographic antenna (trade mark EQVILUM).

A series of experiments were carried out to determine the number of double-strand breaks in DNA (DS DNA) by the foci of γH2AX repair proteins, to assess proliferative activity and clonogenic ability after 3 hours of exposure to microwave electromagnetic radiation with a frequency radiation of 1200 MHz both with shielding and without it on a culture of human lung fibroblasts (MRC5 line). It is shown that no changes in the level of DS DNA were revealed in experimental cells, however, a decrease in the clonogenic ability of irradiated cells without shielding was observed against the background of their high proliferative activity. The mechanisms of the observed cellular effects formation are unclear and we suppose that they can be associated with both a change in the metabolism of irradiated cells and with the triggering of certain intracellular mechanisms initiated by electromagnetic radiation (EMR).

This study made it possible to identify effective ways of integral assessment of the biological effects of microwave electromagnetic radiation as well as ways of protection against it. A film microcircuit – an autonomous module of a passive multi-frequency re-emitter generator with a radio holographic antenna (EQVILUM trademark) has shown its high efficiency in protection against man-made microwave electromagnetic radiation and can be recommended as an additional means of effective protection.

Pages: 21-29
For citation

Guryev D.V., Smetanina N.M., Rodneva S.M., Tsishnatti A.A., Molodtsova D.V., Fedotov Yu.A., Dmitriev A.S., Artamonov A.A.

Biological effect of microwave electromagnetic radiation: methods of registration and protection. Biomedicine Radioengineering. 2021.  V. 24. № 2. Р. 21−29. DOI: https://doi.org/10.18127/j15604136-202102-03 (in Russian).

References
  1. Zubarev Yu.B. Mobilnyy telefon i zdorovye: Monografiya. M.: Biblio-Globus. 2019. 234 s. DOI: 10.18334/9785907063312 (in Russian).
  2. Puck T.T., Marcus P.I., Cieciura S.J. Clonal growth of mammalian cells in vitro: growth characteristics of colonies from single HeLa cells with and without a" feeder" layer. The Journal of experimental medicine. 1956. V. 103. №. 2. P. 273–284.
  3. Rafehi H., Orlowski C., Georgiadis G.T., Ververis K., El-Osta A., Karagiannis T.C. Clonogenic assay: adherent cells. Journal of Visualized Experiments. 2011. № 49. P. e2573. DOI: 10.3791/2573
  4. Grekhova A.K., Pustovalova M.V., Eremin P.S., Yashkina E.I., Osipov A.N. Problema analiza postradiatsionnykh izmeneniy kolichestva fokusov γN2AX v asinkhronnoy kletochnoy populyatsii. Radiatsionnaya biologiya. Radioekologiya. 2018. T. 58. № 5. S. 484–489. DOI: 10.1134/S0869803118050077 (in Russian).
  5. Dmitriyev A.S., Itskov V.V., Ryzhov A.I., Grigoryev O.A. Eksperimentalnaya aprobatsiya personalnogo dozimetra mikrovolnovogo elektromagnitnogo izlucheniya «Mera». Zhurnal radioelektroniki. 2020. № 7. DOI https://doi.org/10.30898/1684-1719.2020.7.7 (in Russian).
  6. Dmitriyev A.S., Itskov V.V., Ryzhov A.I., Uvarov A.V. Mikrovolnovaya elektromagnitnaya dozimetriya personalnogo ekologicheskogo prostranstva. Fizicheskiye osnovy priborostroyeniya. 2020. T. 9. № 1. S. 85–99. DOI: 10.25210/jfop-2001-085099 (in Russian).
  7. de Pomerai D., Daniells C., David H., Allan J., Duce I., Mutwakil M., Candido P. Non-thermal heat-shock response to microwaves. Nature. 2000. V. 405. № 6785. P. 417–418. DOI: https://doi.org/10.1038/35013144
  8. Czyz J., Guan K., Zeng Q., Nikolova T., Meister A., Schönborn F., Wobus A.M. High frequency electromagnetic fields (GSM signals) affect gene expression levels in tumor suppressor p53-deficient embryonic stem cells. Bioelectromagnetics: Journal of the Bioelectro-magnetics Society, The Society for Physical Regulation in Biology and Medicine, The European Bioelectromagnetics Association. 2004. V. 25. №. 4. P. 296–307. DOI: https://doi.org/10.1002/bem.10199
  9. Leszczynski D., Joenväärä S., Reivinen J., Kuokka R. Non-thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: molecular mechanism for cancer-and blood-brain barrier-related effects. Differentiation. 2002.  V. 70. № 2-3. P. 120–129. DOI: https://doi.org/10.1046/j.1432-0436.2002.700207.x
  10. De Iuliis G.N., Newey R.J., King B.V., Aitken R.J. Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro. PloS one. 2009. V. 4. № 7. P. e6446. DOI: https://doi.org/10.1371/journal.pone.0006446
  11. Friedman J., Kraus S., Hauptman Y., Schiff Y., Seger R. Mechanism of short-term ERK activation by electromagnetic fields at mobile phone frequencies. Biochemical Journal. 2007. V. 405. № 3. P. 559–568. DOI: https://doi.org/10.1042/BJ20061653
  12. Diem E., Schwarz C., Adlkofer F., Jahn O., Rüdiger H. Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2005. V. 583. № 2. P. 178–183. DOI: https://doi.org/10.1016/j.mrgentox.2005.03.006
  13. Phillips J.L., Singh N.P., Lai H. Electromagnetic fields and DNA damage. Pathophysiology. 2009. V. 16. № 2-3. P. 79–88. DOI: https://doi.org/10.1016/j.pathophys.2008.11.005
  14. Markovà E., Malmgren L.O.G., Belyaev I.Y. Microwaves from mobile phones inhibit 53BP1 focus formation in human stem cells more strongly than in differentiated cells: possible mechanistic link to cancer risk. Environmental health perspectives. 2010. V. 118. №. 3.  P. 394–399. DOI: https://doi.org/10.1289/ehp.0900781
  15. Belyaev I.Y., Hillert L., Protopopova M., Tamm C., Malmgren L.O., Persson B.R., Harms-Ringdahl M. 915 MHz microwaves and 50 Hz magnetic field affect chromatin conformation and 53BP1 foci in human lymphocytes from hypersensitive and healthy persons. Bioelectromagnetics. 2005. V. 26. № 3. P. 173–184. DOI: https://doi.org/10.1002/bem.20103
Date of receipt: 11.10.2020
Approved after review: 25.10.2020
Accepted for publication: 26.02.2021