350 rub
Journal Biomedical Radioelectronics №2 for 2020 г.
Article in number:
Techniques for investigating the electromagnetic sensitivity of biological systems
DOI: 10.18127/j15604136-202002-01
UDC: 621.372.8
Authors:

K.D. Kazarinov – Ph.D. (Biol.), Senior Research Scientist, Leading Research Scientist,  Kotelnikov Institute of Radio Engineering and Electronics of RAS, 

Fryazino Branch (FIRE RAS) (Fryazino, Moscow region)

E-mail: kazarinovkonstantin@yandex.ru

Abstract:

Statement of the problem. The conditions of a biological experiment and the goals of research dictate the requirements for a fundamental rationalization of traditional experimental methods and devices for their implementation. In addition, in the process of biological research, as a result of adaptation of the known methods, it is necessary to create new biophysical methods and devices.

Aim of the work. Development of methods and instruments for biophysical diagnostics that can be used to study the effects of microwave radiation on biological and model systems.

Results. The method of acoustic detection of absorbed power in the millimeter wavelength range was developed. Based on this method, results of experimental skin research were obtained. An experimental method has been developed to investigate the surface properties of lipid monolayers to study microwave biological effects and the results of its implementation in the field of physical chemistry are presented. A new equipment and a waveguide-dielectric method for studying liquids with high absorption capacity are presented. The biological effect of microwave radiation is given using the developed device and an effective chemiluminescent method for the analysis of fast biochemical reactions accompanying the formation of free radicals, as well as a method for measuring the rate of transepithelial water flow through human skin.

Practical significance. The new device and methods for studying the biological effects of microwave radiation presented in the article can be useful for a wide range of specialists in the field of bioelectromagnetobiology.

Pages: 5-14
References

​​​​​

  1. Vlasova I.I., Mikhalchik E.V., Gusev A.A., Balabushevich N.G., Gusev S.A., Kazarinov K.D. Extremely high frequency electromagnetic radiation promotes neutrophil antimicrobial activity. Bioe-lectromagnetics. February 2018. V. 39, Is. 2. P. 144–155.
  2. Kazarinov K.D., Marechek S.V., Polnikov I.G. Voprosy izmereniya pogloshchennoj moshchno-sti mikrovolnovogo izlucheniya v biofizicheskom eksperimente. Elektronnaya tekhnika. Ser. 1 Tekhnika SVCH. 2015. Vyp. 2(525). S. 59–70 (In Russian).
  3. Gapeev A.B. Chemeris N.K. Voprosy dozimetrii pri issledovanii biologicheskogo dej-stviya elektromagnitnogo izlucheniya krajne vysokih chastot. Biomedicinskaya radioelektronika. 2010. № 1. S. 13–35 (In Russian).
  4. Furia L. Hill D. W., Gandhi Om P. Effect of Millimeter-Wave Irradiation on Growth of Sac-charomyces cerevisiae. IEEE Nransations on Biomedical Engineering. 1986. V. BME-33. № 11. P. 993–999.
  5. Polnikov I.G., Gerasimov V.V., Kazarinov K.D. Issledovanie KVCH pogloshcheniya biologi-cheskih rastvorov i preparatov metodom fotoakusticheskoj spektroskopii. Elektronnaya tekhni-ka. Ser. 1. SVCH-tekhnika. 2009. № 4. S. 59–65 (In Russian).
  6. Kazarinov K.D., Polnikov I.G. Mikrovolnovoe izluchenie i kozha cheloveka. Razrabotka neinvazivnyh metodov issledovaniya. Elektronnaya tekhnika. Ser. 1. SVCh-tekhnika. 2018. Vyp. 4 (539). S. 57–65 (In Russian).
  7. Titov S.V., Malinin V.S., Titov A.S., Kazarinov K.D. Issledovaniya poverhnostnyh svojstv lipidnyh monosloev – modelej biologicheskih membran. Zhurnal radioelektroniki [elektronnyj zhurnal]. 2018. №10. Oktyabr'. S. 1–15 (In Russian).
  8. Titov S.V., Malinin V.S., Titov A.S., Kazarinov K.D. Raschet parametrov generacii ka-pillyarnyh voln v vodnoj srede. Zhurnal radioelektroniki [elektronnyj zhurnal]. 2019. № 1. Yanvar'. S. 1–15 (In Russian).
  9. Kazarinov K.D., Malinin V.S., Polnikov I.G. Izuchenie s pomoshch'yu metoda kapillyarnyh voln dejstviya mikrovolnovogo izlucheniya na poverhnostnye svojstva lipidnyh monosloev – modelej biologicheskih membran. Elektronnaya tekhnika. Ser. 1. SVCH-tekhnika. 2019. Vyp. 1 (544). S. 76–83 (In Russian).
  10. Novgorodceva T.P. Lipidomika v mediko-biologicheskih issledovaniyah NII medicin-skoj klimatologii i vosstanovitel'nogo lecheniya. Zdorov'e. Medicinskaya ekologiya. Nauka. 2009. № 4–5 (39–40). S. 133–137 (In Russian).
  11. Kazarinov K.D., Polnikov I.G. Izuchenie biologicheskogo dejstviya mikrovolnovogo iz-lucheniya s pomoshch'yu hemilyuminescentnogo metoda. Elektronnaya tekhnika. Ser.1. SVCH-tekhnika. 2010. № 2. S. 57–71 (In Russian).
  12. Malinin V.S., Putvinsky A.V., Kazarinov K.D. Calcium dependent Activation of Human Blood Neutrophils Electric Fields Pulses. In: Electricity and magnetism in Biology and Medicine. Plenum publishing corporation. N.Y. 1999. P. 569–572.
  13. Borisenko G.G., Polnikov I.G., Kazarinov K.D. Biologicheskie membrany – pervichnye misheni recepcii elektromagnitnyh polej v medikobiologicheskom eksperimente. Elektron-naya tekhnika. Ser.1. SVCH-tekhnika. 2007. № 4. S. 25–37 (In Russian).
  14. Chekanov A.V., Panasenko O.M. Osipov A.N., Arnhol'd Yu., Kazarinov K.D. Vzaimodej-stvie tret-butilgidroperoksida s gipohloritom privodit k obrazovaniyu perekisnyh radika-lov. Issledovanie metodom hemilyuminesscencii. Biofizika. 2002. T. 47. Vyp. 5. S. 787–794 (In Russian).
  15. Chekanov A.V., Panasenko O.M., Osipov A.N., Kazarinov K.D. Vzaimodejstvie gipohlo-rita s gidroperoksidom zhirnoj kisloty privodit k obrazovaniyu svobodnyh radikalov. Bio-fizika. 2005. T. 50. Vyp. 1. S. 13–19 (In Russian).
  16. Chekanov A.V., Baranova O.A., Levin A.D., Solov’eva E.Yu., Fedin A.I., Kazarinov K.D. In-fluence of gold nanoparticles on activation of human blood neutrophils. Biophysics. May 2013. V. 58. Is. 3. P. 385–388.
  17. Kazarinov K.D., Baranova O.A., Polnikov I.G., Chekanov A.V. Izuchenie vozmozhnosti primeneniya nanochastic v SVCH-onkotermii. Elektronnaya tekhnika. Ser. 1. SVCH-tekhnika. 2017. Vyp. 1 (532). S. 54–64 (In Russian).
  18. Kazarinov K.D., Letyaeva A.V., Polnikov I.G. Issledovanie pogloshcheniya mikrovolnovo-go izlucheniya tonkim polietilenovym kapillyarom, zapolnennym suspenziej liposom. Elektronnaya tekhnika. Ser. 1. SVCH-tekhnika. 2013. № 1 (516). S. 48–54 (In Russian).
  19. A.s. №1101750. Sposob izmereniya moshchnosti SVCh-izlucheniya. O.V. Beckij, K.D. Kaza-rinov, A.V. Putvinskij, V.S. Sharov (In Russian).
  20. Kazarinov K.D., Polnikov I.G. Opyt ispol'zovaniya volnovodno-dielektricheskoj kame-ry dlya izmereniya koncentracii vody v organicheskih sredah i oblucheniya biologicheskih ob"ek-tov. Elektronnaya tekhnika. Ser. 1. SVCH-tekhnika. 2018. Vyp. 3 (538). S. 92–102 (In Russian).
  21. Patent № 2614047 (RF). Perestraivaemaya volnovodno-dielektricheskaya kamera dlya kontrolya zhidkostej. K.D. Kazarinov, I.G. Polnikov (In Russian). 
  22. Patent № 2655028 (RF). Volnovodnoe ustrojstvo dlya izmereniya para-metrov zhidkostej. K.D. Kazarinov, E.A. Tihonova, V.S. Solosin (In Russian).
  23. Patent № 2631340 (RF). Sposob izmereniya koncentracii vodnyh ras-tvorov. K.D. Kazarinov, I.G. Polnikov (In Russian).
  24. Kazarinov K.D., Polnikov I.G. SVCH-izmeriteli vlagosoderzhaniya uglevodorodnyh sme-sej (razrabotka metodov i konstrukcij). Zhurnal radioelektroniki [elektronnyj zhurnal]. 2018. № 8. Avgust. S. 1–19 (In Russian).
  25. Johnsen G.K., Haugsnes A.B., Martinsen O.G., Grimnes S. Stratum corneum in vivo water content from TEWL-measurements. IEEE Eng Med Biol Soc. 2008. P. 3166–3169.
Date of receipt: 20 января 2020 г.