K.V. Ustenko Post-graduate Student, National Research Nuclear University «MEPhI» (Moscow) E-mail: ksustenko@yandex.ru D.V. Uskalova Post-graduate Student, National Research Nuclear University «MEPhI» (Moscow) E-mail: uskalovad@mail.ru E.I. Sarapultseva Dr.Sc. (Biol.), Associate Professor, Obninsk Institute of Nuclear Power Engineering NRNU «MEPhI» E-mail: helen-bio@yandex.ru

Purpose: To analyze the changes in survival, fertility and metabolic activity in the four generations of crustacean Daphnia magna after low-intensity radiofrequency exposure in different periods of ontogenesis. Material and methods: One-day daphnids (generation of F0) were irradiated with frequency of 900 MHz and energy flux density of 100 µW/cm2 in a juvenile period (1−5 days of ontogeny) and period of gustation (6−10 days). After that Daphnia were cultured in climate control incubator to 21-days-old. Generations F1, F2 and F3 were formed from one-day Daphnia and were not irradiated. Dead and newborn daphnids were counted and removed. 5- and 10-days-old crustaceans were used in the MTT-assay. This test integrated the activity of mitochondrial dehydrogenases, the level of free radicals, the ratio of live and dead cells and the activity of antioxidant enzymes. Results: The survival of D. magna, irradiated during the juvenile period, was significant compromised. The effect was maintained in the first generation and reduced in the second and third generations. The fertility of D. magna, irradiated during the juvenile period, was significant decreased only in the F2 generation. The fertility of D. magna, irradiated during the period of gustation, was compromised in the F1 and F2 generations and restored in the third post-radiation generation. Violation of survival in Daphnia correlated with violation of metabolic activity (r = 0.66). Conclusion: Low-intensity radiofrequency radiation can cause long-term effects of viability in D. magna. Irradiation in the early juvenile period reduced the survival of Daphnia in the first generation and compromised fertility in the second generation. Irradiation in the period of gustation reduced fertility in the first and second generations. These effects should be taken into account analyzing the direct and long-term effects of low-intensity radiofrequency irradiation in ecosystems.

 

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