T.M. Sabelnikova Ph.D. (Eng.), Associate Professor, Department of Chemistry, Bauman Moscow State Technical University V.V. Sabelnikov Ph.D. (Eng.), Associate Professor, Department of Machine Building, Bauman Moscow State Technical University V.N. Goryacheva Ph.D. (Chem.), Associate Professor, Department of Chemistry, Bauman Moscow State Technical University E-mail: smitr@mail.ru

The paper mentions experimental results aimed at reinforcement of ultrasonic vibration chemical effect on infected wounds typical microflora (Staphilococcus aureus, Bacillus pynocyaneus, Proteus, Colibacillus) due to combining the low-frequency ultrasound effect with antiseptics of oxidizing group. The performed studies showed that low-frequency ultrasound with operating frequency of f=26.5 kHz and concentrator end displacement amplitude of А= 40-45 μm has bactericidal effect towards all tested microorganisms. However, bacterial flora de-pression is achieved at relatively long sonic treatment ranging from 18 to 27 minutes. In this case negative effect of ultrasound on civilized cells and fibrinous wound sheet is possible. During 5-7 minutes sonic treatment of staphylococcic microflora the number of microbial bodies in the solution doubles or triples compared to original. This fact occurs due to disintegration of bacterial cell cluster during the initial period of ultrasonic exposure. According to relative bactericidal action resistance of low-frequency ultrasonic vibrations the tested types of microorganisms stand in the following sequence: Staphilococcus aureus, Colibacillus, Bacillus pynocyaneus, Proteus. Owing to the conducted experiments cavitation proved to be a determining factor in the bactericidal effect of low-frequency ul-trasound. In order to reduce the time necessary for sonic treatment and to save high bactericidal effect it is suggested to intensify the chemical effect of ultrasound by antiseptics of weak concentration. The best efficiency was stated to be achieved when using hydrogen peroxide solution as antiseptic. This fact allows reducing the time necessary for sterilization of tested bacterial suspensions from 1.7 to 2.0 times. The results of the performed studies were used in developing a new method of ultrasonic treatment of infected wounds at the department of Biomedical Technologies (BMT-1) in BMSTU. The method proposed was patented in the Russian Federation and successfully tested in Central Military Clinical Hospital named after N.N. Burdenko.

 

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