S.D. Solnushkin - Senior Research Scientist, Pavlov Institute of Physiology RAS, St.-Petersburg. E-mail: solnushkin@list.ru V.N. Chikhman - Ph.D. (Eng.), Head of Information Technology Laboratory, Pavlov Institute of Physiology RAS, St.-Petersburg. E-mail: niv@pavlov.infran.ru

The program Impuls to study the pharmacology of synaptic transmission in the vestibular receptors of the frog in neuroexperiments is developed. In the experiments a control of frequency changes of neuronal activity is performed with simultaneous recording of multiple (up to ten) biochemical effects on the studied biological object. The source of the experimental data for the program Impuls is designed early device MD236, to count the pulses generated in a given time interval with the subsequent issuance of the count in a computer via USB. The program Impuls released three modes of operation: «experiment», «results processing», «work with archives». In the «experiment» mode an accumulation of experimental data in real time is carried out. The graphical interface of the program contains fields to display the measurement results of recorded signals and controls to set the measurement parameters. At the initial stage measurement parameters (pulse amplitude threshold, the value of the interval for pulse counting, the value range display the results) are set. After starting the phase of measurement the dynamic display of measurement results with the possibility of fixing the special labels on the current chart changes in the frequency of impulses is performed. Thus possible to identify «areas of interest», reflecting the behavior of registered processes by external influences, such as the introduction of biologically active chemicals (noradrenaline, acetylcholine, and others). Type of drug, concentration is set by the experimenter with a pre-compiled «drop-down lists.» Tags reflect moments of the beginning and end of the pharmacologic effects on the object. The control of graphics during the experiment is provided with sliders - zooming along the axis Y of amplitude values. To control the current pulsation frequency and time from the start of the experiment are displaying in individual graphic windows. The experimental results are stored in the database. The stage of processing the results of the experiment is carried out with a database. There is implemented control charts filtration, separation «areas of interest» with the calculation of local maxima or minima, the area under the curve with respect to a logical zero line. The program was developed in the environment of Delphi v.6.0 with library functions using Windows API, to display graphs component SL Scope of freely distributed library of visual components Mitov software is used.

 

1. Molodcov V.O., Smirnov V.JU., Solnushkin S.D., CHikhman V.N. Ustrojjstvo dlja izmerenija chastoty spajjkov // Pribory i tekhnika ehksperimenta. 2013. № 6. C. 88-89.