S.A. Ivlev - Engineer-developer, Research Institute for Neurocybernetics Academy of Biology and Biotechnology SFU (Rostov-on-Don). E-mail: sergey190654@yandex.ru A.G. Suhov - Dr. Sc. (Biol.), Professor, Research Institute for Neurocybernetics Academy of Biology and Biotechnology SFU (Rostov-on-Don). E-mail: w701@krinc.ru G.G. Bondar - Ph.D. (Biol.), Senior Research Scientist, Research Institute for Neurocybernetics Academy of Biology and Biotechnology SFU (Rostov-on-Don). E-mail: ins270386@yandex.ru

When intracellular recording of neuronal activity must take into account the elasticity of the membrane, complicating controlled dive deep into the microelectrode cells and increases the likelihood of neuronal damage by puncturing the membrane. Typically, the penetration of the cell used neat pressing (soft or, conversely, sharp) of the manipulator Another way is to pass through electrode a high-frequency voltage («Buzz» or «Tickle»), in this case requires strictly dosed of impact to ensure penetration of the microelectrode into the cell and at the same time not damage it. None of these methods is not absolute advantages and can be used by experimenter depending on the particular cell type. To carry out such experiments the less traumatic and more controllable method is more suitable. This method provides impart of a shock acceleration giving a microelectrode the short and dosed amplitude step needed for the penetration through the cell membrane to a predetermined depth. It is desirable that the one-time impact effect on the micropipette was, without further additional vibration. Proposed in this paper miniature device is designed in such a way as to minimize the inevitable uncontrolled vibrations produced by a shock acceleration. A one-time pulse is fed to the controlling electromagnet of the device using the pulse generator. In this case, the shock rod is moved by an electromagnet and carries shock acceleration at the butt of micropipette. The recoil compensation is performed by using a compensating rod, connected the same way as the shock rod through tiny springs to the common point of the device. The compensating rod is driven by an additional electromagnet, the run of which is performed in parallel with the electromagnet controlling the shock rod.

 

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