A.A. Oleshkevich - Ph.D.(Biol.), Associate Professor, Department of Informational Technologies, Mathematics and Physics, Moscow State Veterinary Academy
E.V. Kaminskaya - Senior Research Scientist, Institute of Medical-Biological Problems, Russian Academy of Sciences
A.M. Nosovskiy - Dr.Sc.(Biol.), Leading Scientific. Employee, Institute of Medical-Biological Problems, Russian Academy of Sciences

The effect of ultrasound (US) on leukocytes and on their practically important metabolites was studied. Preliminary studies allowed selecting the interferon (IFN) production stimulating conditions. The optimal mode of physical impact was obtained. The possibility of a mathematical model constructing was tested. The results obtained were evaluated by mathematical analysis and mathematical modeling to predict the long-term response of suspension cultures and to control the functional state of the cells in response to acoustic (ultrasonic) waves. The use of analysis of variance for predicting the yield of protein production explored. There was no cell disruption after treatment with ultrasonic intensity of from 0.01 to 0.05 W/cm2, exposure at an intensity of 0.1 W/cm2 resulted in decreased viability of leukocytes 10.6%. After treatment with ultrasound intensity 0.2-2.0 W/cm2 number of viable cells decreased even more significantly. Fig.1 shows that the intensity increases from 0.1 to 0.4 W/cm2, the number of viable cells is reduced. When ultrasonic action with 0.4-0.7 W/cm2 intensity remains constant level of viable cells in suspension. Higher ultrasonic intensity causes a sharp reduction in the viability of leukocytes in the suspension due to cavitations threshold of which depends on the cell concentration in the bulk medium and diluted suspensions coincides with the threshold of cavitations in water ~ 0.3 W - cm 2. However, about 45 % of the cells do not die, they remain viable . Aggregation of leukocyte cells . After ultrasonic influence cell suspension mikroskopirovali and studied morphology changes of leukocytes. It was found that the number of agglomerates formed during 5 minutes before exposure to US, the intensity increases with ultrasonic.At low US intensities (0.01-0.1 W/cm2) the associations of 4-8 cells were detected. At higher doses ultrasonic observed accumulation of a plurality of cells up to clot. Seen in micrographs intense cell aggregation , since doses of 0.7 W × cm- 2, and more. The dispersion state of blood cells is provided by the electrostatic forces of repulsion between like-charged cell membranes. The phenomenon of aggregation of white blood cells when exposed to US may explain the loss of cell surface charge and "gluing" of the membranes. It has been found that when cells were treated with low US intensity leykoformula changes slightly. At higher doses of US cell aggregation occurs. Basically agranulocytes aggregated, particularly lymphocytes (leykoformula shifted to the left), i.e. the number of neutrophils in the field of microscopy increases.