A.V. Sergutina - Ph.D. (Biol.), Senior Research Scientist, Research Center of Neurology Russian Academy of Medical Sciences, Moscow. E-mail: sergutina.anven@yandex.ru
V.I. Rakhmanova - Programmer, Research Center of Neurology Russian Academy of Medical Sciences, Moscow

In the present work, the effect of delta-sleep-inducing-peptide (DSIP) on changes in activities of acetylcholine esterase (AChE) and monoamine oxidase (MAO - substrates serotonin and triptamine) induced by L-DOPA in brain of rats with different emotional reactivity was studied. DSIP was investigated as stress-correcting substance. Wistar rats with high level of motor activities (emotionally stable) and August rats with low level of motor activities (emotionally responsive) were compared. Quantitative cytochemical methods were used for measurement of AChE and MAO (substrates serotonin and triptamine) activities in sensorimotor cortex (layers III and V), n. caudatus, n. accumbens, and hippocampus (CA3 field). The study groups included control animals (administration of physiological solution) and those receiving intraperitoneal injection of Madopare-125 (25,5 mg/kg body mass per day during 2 weeks) which induced hyperfunction of dopaminergic system. DSIP was applied in vivo in dose of 60 mkg/kg body mass following Madopare-125 administration. We have undertaken a morphochemical investigation of the brain of August and Wistar rats in control (n=6) and experimental (n=6) groups of animals. Statistical analysis was performed using and one-factor dispersion analysis (One-way ANOVA). Wistar rats. It was shown that L-DOPA administration resulted in the decrease AChE activity in n. caudatus and MAO (triptamine) activity in layer III of sensorimotor cortex. In response to L-DOPA the activity MAO (triptamine) increased in hippocampus. Administration of DSIP following L-DOPA did not restore the activity of AChE in n. caudatus, but increased it in layer III of sensorimotor cortex. The activity of MAO (triptamine) decreased in layer III of cortex, returned to normal level in layer V of cortex, increased in n. caudatus and n. accumbens, but it remained higher than control level in hippocampus. The activity of MAO (serotonin) increased in cortex, n. caudatus and n. accumbens as compared to control level. August rats. L-DOPA administration resulted in the decrease AChE activity in n. caudatus, the increase MAO (triptamine) activity in n. caudatus and hippocampus, the increase activity MAO (serotonin) in cortex, n. caudatus and n. accumbens as compared to control level. Administration of DSIP following L-DOPA decreased the alterations of AChE and MAO (serotonin and triptamine) activities induced by L-DOPA. AChE activity increased in n.caudatus. Thus, the study allowed to reveal the morphochemical peculiarities of AChE and MAO activities in brain of August and Wistar rats changes in response to L-DOPA and after DSIP administration.