T.A. Sakharnova - Junior Research Scientist, NNSMA, Nizhny Novgorod, Russia. E-mail: saHarnova87@mail.ru
M.V. Vedunova - Ph.D. (Biol.), Senior Research Scientist, Lobachevsky State University of Nizhni Novgorod, Russia. E-mail: MVedunova@yandex.ru
E.V. Mitroshina - Junior Research Scientist, NNSMA, Nizhny Novgorod, Russia. E-mail: Mitroshinae@list.ru
I.V. Mukhina - Dr.Sc. (Biol.), Professor, NNSMA, Nizhny Novgorod, Russia; Lobachevsky State University of Nizhni Novgorod, Russia

Nowadays investigations concerning the mechanisms of hypoxia and ways of its therapeutic correction are the one of the priority area in neuroscience and medicine. Neurotrophic factors BDNF and GDNF are considered as endogenous neuroprotective substances which potentially minimize the possibility of the development of pathological processes associated with oxygen starvation of the brain. It was shown neurotrophic factors BDNF and GDNF to be actively participate not only in the neurogenesis, but can also correct the metabolism of mature neurons [1,2]. However, a question of the role of BDNF and GDNF in the regulation of oxidative processes in mature brain remains unclear. The aim of the investigation was to study the estimation of neurotrophic factor-s (BDNF and GDNF) influence on the animal survival, the preservation their motor activity and long-term memory during acute hypobaric hypoxia and in the posthypoxic period. The experimental subjects were 64 adult male mice (line C57BL/6) weighing 18-20 grams. Before modeling of acute hypoxia the animals tested by the «Open field» method and passed the navigation training in «Morris water maze». Then mice were placed in conditions corresponding altitude10000-10500 m (170-185 mm Hg) with a lifting speed 183 m/s. The main parameters were registered: a lifetime on the "height"; a time of loss and recovery physiological posture. Moreover in each group there were an evaluation of the individual stability to acute hypoxia and calculation of the protection coefficient. The following day after hypoxia the animals were re-tested in the «Open field» and determined deferred long-term memory retention rate in «Morris water maze». Carry out experiment reviled preventive intranasal injection of BDNF and GDNF (4 mkg/kg, 40 mkg/kg) to increase the resistance of mice to acute hypoxia and the restoration of motor activity and preservation of spatial memory in the posthypoxic period. That allows us to consider BDNF and GDNF as important signaling molecules having not only neuroprotective, but also antihypoxic properties. The research was supported by grants of Russian Foundation of Basic Research № 13-04-01871, № 13-04-12067, № 14-04-31601.