A.A. Oleshkevich - Ph.D.(Biol.), Associate Professor, Department of Informational Technologies, Mathematics and Physics, Moscow State Veterinary Academy
A.M. Nosovskiy - Dr.Sc.(Biol.), Leading Scientific. Employee, Institute of Medical-Biological Problems, Russian Academy of Sciences
E.V. Kaminskaya - Senior Research Scientist, Institute of Medical-Biological Problems, Russian Academy of Sciences

It was shown that low-intensity ultrasound 0.05-0.1 W/cm2 with an exposure time of 1-3 minutes, almost no effect on the subsequent growth and development of bacterial cultures A .fischeri strain MSU 6 , lowered the intensity of luminescence , " dubbed " cells . However, the intensity of the glow is quickly restored after the termination of the CL. When the intensity of ultrasound from 0.1 to 0.2 W/cm2 emission level cells increases and reaches the reference. The impact of ultrasound intensity of 0.4 W/cm2 has a stimulating effect on bioluminescence and to a lesser extent by an increase in the rate of growth A.fischeri. In the range between 0.4-0.6 W/cm2 visible changes bacterial luminescence was observed. It is obvious that under these intensities effects of suppression and stimulation of bioluminescence exist both. At intensities greater than 0.6 W/cm2 , there is an irreversible inhibition of bioluminescence. The number of viable cells was progressively reduced under intensity of ultrasound when the inhibition of bioluminescence coincides with the cavitation threshold. This assumption is supported by experiments in which it was shown that under our experimental conditions of ultrasonic cavitation occurs when the intensity of illumination of 0.6-1.0 W/cm2 in a medium free from bacterial cells.Analysis of the data indicates that the biological effects of ultrasound thresholds, expressed in a change / stimulation / parameters that characterize the metabolic rate (increased bioluminescence , the acceleration of growth) in luminous bacteria A.fischeri, well below the threshold of ultrasonic destruction of cells in suspension.It was also studied the change in the luminescence dynamics of bacterial growth A.fischeri after exposure to ultrasound optimal intensity of 0.4 W/cm2 on the initial suspension of cells used to inoculate the culture medium (i.e. in the inoculum ). It is clearly seen that as a result of the stimulating effects of ultrasound maximum intensity of the luminescence of cells in the experimental apparatus was higher than the control by about 35 % -45 %. The combined effect of high-frequency ultrasound (0.4 W/cm2intensity , exposure time 5 min) and drugs used for the treatment of malignant tumors was studied. Doxorubicin , broad-spectrum antibiotic , and cyclophosphamide, cytostatic agent , were added to culture flasks at the rate of 0.8 mg / ml of the initial medium and 0.2 mg / ml, respectively.Studies have shown that ultrasound enhances the suppressor effect of antibacterial drugs on the cell, slows its growth, causes a dramatic reduction in the number of viable cells , reducing the emission intensity , allows to reduce the dose of the drug introduced , not only did not weaken the effect, and vice versa , strengthening it, causing inhibition metabolic processes and cellular division. This property can be used as a rapid analysis of the effects of newly synthesized drugs.