Postnov S.E., Mezentseva M.V., Podchernyaeva R.Ya., Danlyubaeva G.A., Surgucheva I.M., Grinkevich O.M., Lopatina O.A., Baklanova O.V.

The purpose: Examination of biological activity of boundary water (AWODA), explaining its biological mechanism effect on living organism. It was shown in experiments in vitro, that: 1.While cultivation with addition of normal cells of AWODA (4 lines), proliferation and viability of cell-s cultures practically doesn-t change. As in cancerous cells (6 lines), we noted proliferate activity depression in the passing process. Insignificant changes in normal lines and total degeneration in 3 of 6 lines of oncogenic cells were shown in morphological researches. 2.AWODA addition to toxic serum of ECS (embryonic calf serum) leads to their toxicity decreasing and they can be used for cells cultivation and cells biomass following application for bioengineering target and molecular-biological studies. 3.As a substance, AWODA has a marked antivirus effect on human influenza A virus (H3N2), herpes simplex virus types 1 and 2, mouse encephalomyocarditis (EMC) virus. Here, AWODA potentiates the effect of immunomodulatory drugs. Normally, it has interferon-inducing and cytokine-modulating activity and in experimental infection, provoked by influenza virus and herpes simplex virus types 1 and 2. Conducted experiments show a potential possibility to apply AWODA for treating such diseases as urogenital diseases, genital herpes, Hepatitis C virus, influenza and acute respiratory virus infections (ARVI), papilomatosis and tumors, by individual selection for each patient. Experiments in vivo shows: - AWODA, taking in treatment regimen per os, protects from mouse encephalomyocarditis (EMC) virus infection no less then 60 % of animals (in control, < 20% of animals survived) - AWODA, taking in treatment regimen per os, protects from influenza virus infection (H3N2) 60 % of animals (in control, < 20 % of animals survived) - AWODA, taking in treatment regimen introperitoneum, protects from herpes virus (type 1) infection 70 % of animals (in control, < 20 % of animals survived) - Normally, AWODA has a cytokine-modulating activity in vivo. It also has this type of activity in experimental infection, provoked by influenza virus and the herpes simplex virus types 1 and 2, controlling both cellular and humoral animal-s immunity.