S.A. Polina - Post-graduate Student, Institute of Non-ferrous Metals and Material Science, Chemistry Department, Subdepartment of Analytical and Organic Chemistry, Siberian Federal University (Krasnoyarsk). E-mail: Demi-2704@yandex.ru N.E. Khmarskaya - Student, Institute of Non-ferrous Metals and Material Science, Chemistry Department, Subdepartment of Analytical and Organic Chemistry, Siberian Federal University (Krasnoyarsk). E-mail: tafka15@mail.ru A.A. Efremov - Dc.Sc. (Chem.), Professor, Academician of International Academy of Ecology, Man and Nature safety, Head of Laboratory of Chromatographic Analysis Methods, Center for Equipment Joint Use, Siberian Federal University (Krasnoyarsk). E-mail: AEfremov@sfu-kras.ru

It is known that anthocyanins are effective antioxidants and neutralize free oxygen radicals; have decongestant effect and reduce the permeability of the capillaries; have a protective effect on blood vessels, reducing capillary fragility and have a positive effect on vascular lesions; contribute to the stabilization of the connective tissue, normalizing the outflow of intraocular fluid in glaucoma and intraocular pressure. There are numbers of papers dedicated to the study of anthocyanins bilberry. In the main papers used the methods TLC and electron spectroscopy methods are used. But there is not information about anthocyanins of bilberry growing in Krasnoyarsk krai. In this regard, the aim of this work was to find the optimal extraction conditions and determine the composition of anthocyanins in bilberry growing in Krasnoyarsk krai by HPLC. Anthocyanins composition of frozen fetuses bilberry (Vaccinium myrtillus L.) which grows in Yenisei district of Krasnoyarsk krai was investigated by HPLC method with UV detection. There were prepared fifty ethanolic extracts of berries during the experiment, 5 of which infused in 40% ethanol containing 1% HCl at room temperature for 15, 30, 45, 60, 90 minutes at a ratio of feed: extractant 1:30. 45 extracts obtained by heating on a boiling water bath for 15, 30, 45, 60 and 90 minutes with the ratio of feed: extractant 1:30, 1:50 and 1:100. We used 40%, 60% and 95% ethanol with addition of c 1% HCl for extraction. There were found the conditions for optimal extraction of anthocyanins with different concentrations of ethanol (extraction on a boiling water bath for 15 minutes and 40% ethanol containing 1% HCl and ratio of raw materials: extractant 1:30). Maximum cumulative quantitative recovery of this method was 3764.69 ± 0.14. We quantified the content of anthocyanins based on the delphinidin-3-glucoside 9.85 ± 0.08%. Anthocyanin composition of bilberry berries growing in the Krasnoyarsk krai defined by HPLC using a UV detector.

 

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