A.A. Ermoshin - Post-graduate Student, Ural Federal University, Ekaterinburg. E-mail: ermosh@e1.ru L.G. Babeshina - Dr.Sc. (Biol), Professor, Moscow State Regional Humanitarian Institute, Orekhovo-Zuyevo A.P. Kolokolova - Business Partner , Teva Pharmaceutical Industries Ltd.

Members of the genus Sphagnum L. are known in folk and official medicine. They contain polysaccharides (from 9.5 to 26.0%). Quantitatively dominates fraction of pectin which has high antimicrobial activity and sorption. Sorption ability of sphagnum mosses can be associated with polysaccharides, and also with the contained proteins. Information about the qualitative and the quantitative compositions of the proteins of sphagnum mosses is not found in the literature. Seven types of sphagnum mosses from 3 sections were studied. The extraction of soluble proteins was performed in 0.05 M borate buffer for one day under a continuous agitation. The determination of protein was carried out by the Bradford method spectrophotometrically. Studies of soluble protein in turf sphagnum S. balticum in different months showed that the highest content of protein is observed in samples harvested in May (3,73 ± 0,18 mg/g dry weight). In June and July the protein content was reduced (3,19-3,34 mg / g dry weight), and in August was the lowest protein content (2,35±0,13 mg/g). For S. fuscum was shown a different trend: in the beginning of summer protein content falls slightly in comparison with May samples, however in August protein content coincides with the content of protein in May (4,36±0,08 and 4,40±0,11 mg/g dry weight, respectively). We divided the results of determining the amount of protein in 7 species of the genus Sphagnum into 3 groups. The most protein content was detected by S. fuscum (4,4±0,11 mg/g dry weight). The second group consists of species that contain significantly less protein than brown sphagnum and statistically indistinguishable between them (S. papillosium, S. magellanicum, S. balticum, S. russowii: 3,56-3,05 mg/g dry weight). Then follows S. angustifolium (2,32±0,17 mg/g), and the smallest content of the extracted protein is shown for S. fallax (1,71 ± 0,09 mg/g dry weight). Obtained are compared groups in protein content with botanical sections showed no sign of group membership. According to the research was carried out a correlation analysis of the dependence the sorption properties of mosses of the content of protein in the different months of the growing season. For S. balticum and S. fuscum was showed a weak positive correlation (0.4 and 0.5 respectively).

 

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