M.B. Yakovleva, Z.K. Nikitina

The influence of quantity (5 %, 7 % and 10 %) two-days of vegetative sowing material was investigated in work on growth, level activity of extracellular collagenase and proteinase from submerged cultivation P. chrysogenum. The vegetative sowing material cultivated on standard medium Chapek. Then mycelium transfer in modification nutrient medium with substitution of 2 % collagene. The tests take on 3, 4, 5, 6, 7 and 10 days of growth. The cultural liquid separated of filtration after membrane filter with diameter pore 0,09 mkm. The quantity of proteins, general and specific of collagenolytic and proteolytic activity was investigate in test. The most quantity biomass was in variant with 7 % mycelium. The maximum proteinase activity P. chrysogenum was discovered in experiments with 5 % vegetative sowing material. The level of collagenase activity P. chrysogenum was more high on 6 day growth with use the same quantity of vegetative sowing material. The 2 day vegetative mycelium used in quality inoculums broth more advantage for secretion of collagenase and proteinase P. chrysogenum in comparison of spore material. The level of synthesis of proteinase and collagenase of deiteromycetes may be place of regulation introduce of different quantity inoculums.