350 rub
Journal №8 for 2012 г.
Article in number:
Acidophycation Activity of Penicillium Citrinum in the Conditions of Growth on the Zinc- and Copper-Containing Media
Keywords: organic acids nitrogen sources concentration of carbon substrate zinc copper extracellular detoxification adaptation
Authors:
K.V. Barinova, S.M. Schiparev
Abstract:
The aim of this work was to study the influence of Zn and Cu on the growth and organic acid production by fungi on the media with different sucrose concentration and different nitrogen sources. The object of these study Penicillium citrinum L4/09 was isolated from disrupted marble. Organic acid analysis was carried out by chromato-mass-spectrometry on the Agilent MSD5975. It was demonstrated that C/N ratio in the nutrient medium is very important for growth of fungi. High sucrose concentration and only nitrate form of nitrogen promote organic acid formation by P. citrinum L4/09. As a result of the investigation of zinc and copper influence on the growth and development of P. citrinum L4/09 was found that these metals significantly influence on these characteristics of fungi. Zinc promotes the growth of mycelium. Copper in high concentration has some toxic effect on the fungal growth, especially on the poorest medium. It was established that oxalic acid play the specific role in the adaptation of P. citrinum L4/09 by Zn and Cu presence in the medium. The means of adaptation P. citrinum L4/09 by both metals associated with hyper production of oxalate realizes primarily on the poorer media. On the richer nutrient media apparently other adaptive mechanisms takes place.
Pages: 48-56
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