350 rub
Journal №10 for 2012 г.
Article in number:
DNA Visualization During Gel Electrophoresys by Fluorescent Dye Sybr Gold According to Prestaining Protocol
Keywords: DNA visualization SYBR Gold cyanine dye electrophoretic mobility
Authors:
N.Yu. Karpechenko, V.K. Gasanova, N.V. Ryadninskaya, E.A. Lesovaya, K.I. Kirsanov, G.A. Belitsky, M.G. Yakubovskaya
Abstract:
Nonsymmetrical cyanine dye SYBR Gold visualized DNA more efficiently as compared to ethidium bromide and other SYBR dyes. It exerts high affinity to DNA, however, it could be easily removed from the biopolymer by standard ethanol precipitation. SYBR Gold does not alter different molecular biology reactions. Unfortunately, application of the SYBR Gold for DNA visualization using post-staining protocol requires its intensive consumption, while DNA pre-staining causes DNA retardation in gel electrophoresis. We demonstrated dye-DNA ratio threshold under which the dye does not influence DNA electrophoretic mobility. High stability of the dye-DNA complexes was demonstrated. When the dye-DNA ratio exceeds the threshold, retardation effect of SYBR Gold in electrophoresis depends on the DNA amounts in samples.
Pages: 69-73
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