M.N. Bogachuk, O.I. Perederyev, G.V. Ramenskaya

Due to insufficient intake of vitamins with food, needs additional intake of vitamins sources as vitamin- drugs and dietary supplements. For last six years constantly increases range of vitamin and multivitamin drugs was registered. Composition of different vitamin in drugs can change at different times of the year and the people age. So, needs vitamins drugs standardization. Of greatest interest for our study represent a combination of water-soluble vitamins, namely: В1, В2, В6, В12, РР, В5, В9, С, Н (В8). For qualitative and quantitative assay of vitamins in multivitamin preparations in Russia uses different methods of analysis, intend mostly to the study of individual vitamins like: titration, spectrophotometry, polarography, fluorimetry, photoelektrokolorimetriya. These methods do not allow to identify all of the vitamins at one study. Capillary electrophoresis is a relatively new method that allows a lower cost to solve this problem. It is suitable for the separation of different samples and complements the classical separation techniques, such as high performance liquid chromatography and gas chromatography. The method is based on the principle of different rates of migration of charged particles and molecules in static electric field. The aim of our study was to develop a method for qualitative and a quantitative determination of water-soluble vitamins (thiamine chloride hydrochloride, riboflavin, nicotinic acid and nicotinamide, pantothenic acid, pyridoxine hydrochloride, folic acid, ascorbic acid, biotin) by capillary zone electrophoresis in vitamin - mineral complexes. Electrophoretic separation was performed on a system of capillary electrophoresis Agilent 3D CE, DAD, quartz capillary HPCE stndrd cap 64 cm, 50 µm, thermostat temperature of 25 ° C, the effective voltage of 30 kV. Total analysis time 25 minutes. Detection was performed at wavelengths of 210, 254 and 292 nm. Injection was carried out hydrodynamically (50 mbar for 10 s). As a buffer solution using 50 mM borate buffer with 10 % acetonitrile. During the research we have studied various parameters for separation and determination of water-soluble vitamins. Were selected best condition for separation and quantitative determination of all water-soluble vitamins by capillary zone electrophoresis in a single analysis. Validation of the developed method was carried out on the following parameters: selectivity, lower and upper limit of quantitation, accuracy, precision, specificity, linearity and the analytical area. The method developed can be used in pharmaceutical analysis of vitamin preparations.