350 rub
Journal №8 for 2011 г.
Article in number:
Dose-dependent action of some guanidine derivatives on glutathione peroxidase/glutathione reductase antioxidant system and NADPH-generated enzymes at ischemia-reperfusion of rats brain
Keywords: brain ischemia-reperfusion biguanides rats
Authors:
O.V. Suhoveeva, T.N. Popova, A.V. Makeeva, A.A. Agarkov, L.F. Panchenko
Abstract:
In this work of male white laboratory rats were used how object of investigations. Ischemia of animals brain was indused with help occlusion of both total carotid arteries during 30-minute's. Reperfusion was reach by declamping. Blood serum and brain homogenate was used for investigations. Dose-dependent action of some guanidine derivatives (N-[imino(4-morpholinyl)methyl]guanidine (IMMG) and N-[imino(1-piperidinyl)methyl]guanidine(IPMG)) on glutathione antioxidant system functioning and some activities of NADPH-generated enzymes at ischemia-reperfusion of rats brain has been studied. Development of ischemia-reperfusion of rats brain was accompanied by increase of glutathione reductase and glutathione peroxidase specific activities in 3.3 and 1.9 time (in brain), and 2.8 and 1.9 time (in blood serum) accordingly. Furthermore, growth of glucose 6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase specific activities in brain - in 2.9 and 2.2 time, and in blood serum - in 1.8 and 2.2 time accordingly was established. Intraperitoneal introduction of IMMG and IPMG in 25 and 50 mg/kg doses under pathology is accompanied by changes of investigated enzymes activities towards of control values. Probably, action of IMMG and IPMG leading to inhibition of free radical processes, results to decrease of glutathione antioxidant system mobilization. This fact is connected with decrease of NADPH-generated enzymes activities, supplying NADPH for glutathione system. This effect was expressed more less significant at introduction of these biguanides in 12.5 and 75 mg/kg doses under.
Pages: 33-39
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