O.A. Vrzhesinskaya, L.V. Shevyakova, N.N. Makhova, S.N. Zorin, V.M. Kodentsova, V.V. Bessonov

The effect of inclusion of dietary fibers of different origin in the doses corresponding to the maximum allowable level of their intake with food additives in rat diet on microelement assimilation under deep combined deficiency of vitamins has been investigated. The combined deficit of all vitamins was caused by 28-days feeding of Wistar male rats (with an initial body mass 70-80 g) with a semisynthetic diet containing 1/5 part of the level of water- and fat-soluble vitamins in sufficient ration. A deep polyhypovitaminosis has been confirmed by their growth delay, by significantly 2,2 fold reduction of vitamin B1 liver content and some decrease on 18% vitamin B2 liver content, compared with animals treated with adequate diet. Pronounced alimentary vitamin deficiency under adequate content of all minerals in rat diet has been accompanied with a tendency to the increase of iron liver content about 32.7%, but the reduce of copper content 32,0%, and simultaneously had no impact on zinc level. Inclusion of chitosan in rat diet (0.09% of dry feed weight), or wheat bran (0.45%) under combined diet deficiency of vitamins did not affect significantly on the content of iron, copper and zinc in the liver.