Development and validation of methods of quantitative determination hydroxycinnamic acid-s amount in the plant of the Thymus l. Genus
- 1 д.фарм.н., профессор, зав. кафедрой фармакогнозии и ботаники, Курский государственный медицинский университет
- 2 к.фарм.н., доцент, кафедра общей и фармацевтической химии, Медицинский институт, Орловский государственный университет
Abstract
The article presents the results on the development of quantitative methods of determining the amount of hydroxycinnamic acids in the herb of the Thymus L. genus of the Lamiaceae family in terms of rosmarinic acid. The optimum extraction conditions have been identified: fineness of raw material - 2 mm, the extractant - ethyl alcohol 50%, extraction before entering the equilibrium, extraction time - 75 minutes.
Validation of the developed method has been conducted in terms of linearity, repeatability, reproducibility, accuracy.
Total content of hydroxycinnamic acids in the plant Thymus L. genus ranges from 2.62% (Thymus dimorphus Klok.) to 10.83% (Thymus pulegioides L.).
How to cite
Бубенчикова В.Н., Старчак Ю.А. Разработка и валидация методики количественного определения суммы гидроксикоричных кислот в растениях рода тимьян // Вопросы биологической, медицинской и фармацевтической химии. 2015. № 5. С. 14–18.
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