Evaluation of tumor necrosis factor α and interleukin 6 content in parotid salivary fluid of ductal chronic sialadenitis patients

А.S. Aleinikov; I.V. Gaiduk; E.S. Gershtein; A.M. Panin; N.E. Kushlinskii

Evaluation of tumor necrosis factor α and interleukin 6 content in parotid salivary fluid of ductal chronic sialadenitis patients

А.S. Aleinikov¹, I.V. Gaiduk², E.S. Gershtein³, A.M. Panin⁴, N.E. Kushlinskii⁵

research article Pages: 50-56

¹ аспирант, кафедра факультетской хирургической стоматологии и имплантологии, Московский государственный медико-стоматологический университет им. А.И. Евдокимова МЗ РФ
² к.м.н., доцент, кафедра факультетской хирургической стоматологии и имплантологии, Московский государственный медико-стоматологический университет им. А.И.Евдокимова МЗ РФ
³ д.б.н., профессор, лаборатория клинической биохимии, Российский онкологический научный центр им. Н.Н.Блохина РАМН, Москва
⁴ д.м.н., профессор, зав. кафедрой факультетской хирургической стоматологии и имплантологии, Московский государственный медико-стоматологический университет им. А.И. Евдокимова МЗ РФ
⁵ д.м.н., профессор, чл.-корр. РАМН, зав. лабораторией клинической биохимии, Российский онкологический научный центр им. Н.Н. Блохина РАМН

Keywords: saliva ductal sialadenitis TNF-α IL-6

Abstract

18 patients with exacerbation of chronic ductal sialadenitis underwent standard anti-inflammatory complex therapy, and after abatement of acute inflammation salivary fluid was collected. TNF-α and IL-6 content in parotid gland salivary fluid was measured by ELISA techniques. Highly significant concordant increase of TNF-α and IL-6 levels was found in saliva in subacute stage inflammatory phase as compared to remission. IL-6 level was significantly positively associated with disease stage and clinical character of inflammatory process.

How to cite

Алейников А.С., Гайдук И.В., Герштейн Е.С., Панин А.М., Кушлинский Н.Е. Исследование содержания фактора некроза опухоли α и интерлейкина 6 в слюнном секрете околоушной слюнной железы больных хроническим протоковым сиаладенитом // Вопросы биологической, медицинской и фармацевтической химии. 2013. № 4. С. 50–56.

References

Afanasev V.V. Sljunnye zhelezy bolezni i travmy // GEHOTAR-Media. 2012. S. 103 - 163.
Romacheva I.F., JUdin L.A., Morozov A.N. Zabolevanija i povrezhdenija sljunnykh zhelez // Medicina. 1987. 240 s.
Panin A.M., Gajjduk I.V.KHirurgicheskaja stomatologija - vospalitelnye i distroficheskie zabolevanija sljunnykh zhelez. M. Literatura. 2011. S. 126 - 143.
Wang Y., Shnyra A., Africa C., Warholic C., McArthur C. Activation of the extrinsic apoptotic pathway by TNF-alpha in human salivary gland (HSG) cells in vitro, suggests a role for the TNF receptor (TNF-R) and intercellular adhesion molecule-1 (ICAM-1) in Sjogren\'s syndrome-associated autoimmune sialadenitis // Arch. Oral. Biol. 2009. V. 54. № 11. P. 986 - 996.
Matsumura R., Umemiya K., Kagami M., Tomioka H., Tanabe E., Sugiyama T., Sueishi M., Kayagaki N., Yagita H., Okumura K. Expression of TNF-related apoptosis inducing ligand (TRAIL) on infiltrating cells and of TRAIL receptors on salivary glands in patients with Sjogren\'s syndrome // Clin. Exp. Rheumatol. 2002. V. 20. № 6. P. 791 - 798.
Yamakawa M., Weinstein R., Tsuji T., McBride J., Wong D.T., Login G.R. Age-related alterations in IL-1beta, TNF-alpha, and IL-6 concentrations in parotid acinar cells from BALB/c and non-obese diabetic mice // J. Histochem. Cytochem. 2000. V. 48. № 8. P. 1033 - 1042.
Tsuneyama K., Saito K., Ruebner B.H., Konishi I., Nakanuma Y., Gershwin M.E. Immunological similarities between primary sclerosing cholangitis and chronic sclerosing sialadenitis: report of the overlapping of these two autoimmune diseases // Dig. Dis. Sci. 2000. V. 45. P. 366 - 372.
Baggiolini M., Clark-Lewis I. Interleukin-8, a chemotactic and inflammatory cytokine // FEBS Lett. 1992. V. 307. № 1. P. 97 - 101.
Tanaka M., Miyajima A. Oncostatin M, a multifunctional cytokine // Rev. Physiol. Biochem. Pharmacol. 2003. V. 149. P. 39 - 52.
Lee H.M., Cho J.G., Kang H.J., Chae S.W., Hwang S.J., Jung K.Y., Woo J.S. Expression of oncostatin M in chronic obstructive sialadenitis of the submandibular gland // Ann. Otol. Rhinol. Laryngol. 2008. V. 117. № 5. P. 347 - 352.
Cauli A., Yanni G., Pitzalis C., Challacombe S., Panayi G.S. Cytokine and adhesion molecule expression in the minor salivary glands of patients with Sjögren\'s syndrome and chronic sialoadenitis // Ann. Rheum. Dis. 1995. V. 54. № 3. P. 209 - 215.
David T. Wong. Salivary Diagnostics // Wiley-Blackwell. 2008. P. 167 - 174.

[1] Afanasev V.V. Sljunnye zhelezy bolezni i travmy // GEHOTAR-Media. 2012. S. 103 - 163.

[2] Romacheva I.F., JUdin L.A., Morozov A.N. Zabolevanija i povrezhdenija sljunnykh zhelez // Medicina. 1987. 240 s.

[3] Panin A.M., Gajjduk I.V.KHirurgicheskaja stomatologija - vospalitelnye i distroficheskie zabolevanija sljunnykh zhelez. M. Literatura. 2011. S. 126 - 143.

[4] Wang Y., Shnyra A., Africa C., Warholic C., McArthur C. Activation of the extrinsic apoptotic pathway by TNF-alpha in human salivary gland (HSG) cells in vitro, suggests a role for the TNF receptor (TNF-R) and intercellular adhesion molecule-1 (ICAM-1) in Sjogren\'s syndrome-associated autoimmune sialadenitis // Arch. Oral. Biol. 2009. V. 54. № 11. P. 986 - 996.

[5] Matsumura R., Umemiya K., Kagami M., Tomioka H., Tanabe E., Sugiyama T., Sueishi M., Kayagaki N., Yagita H., Okumura K. Expression of TNF-related apoptosis inducing ligand (TRAIL) on infiltrating cells and of TRAIL receptors on salivary glands in patients with Sjogren\'s syndrome // Clin. Exp. Rheumatol. 2002. V. 20. № 6. P. 791 - 798.

[6] Yamakawa M., Weinstein R., Tsuji T., McBride J., Wong D.T., Login G.R. Age-related alterations in IL-1beta, TNF-alpha, and IL-6 concentrations in parotid acinar cells from BALB/c and non-obese diabetic mice // J. Histochem. Cytochem. 2000. V. 48. № 8. P. 1033 - 1042.

[7] Tsuneyama K., Saito K., Ruebner B.H., Konishi I., Nakanuma Y., Gershwin M.E. Immunological similarities between primary sclerosing cholangitis and chronic sclerosing sialadenitis: report of the overlapping of these two autoimmune diseases // Dig. Dis. Sci. 2000. V. 45. P. 366 - 372.

[8] Baggiolini M., Clark-Lewis I. Interleukin-8, a chemotactic and inflammatory cytokine // FEBS Lett. 1992. V. 307. № 1. P. 97 - 101.

[9] Tanaka M., Miyajima A. Oncostatin M, a multifunctional cytokine // Rev. Physiol. Biochem. Pharmacol. 2003. V. 149. P. 39 - 52.

[10] Lee H.M., Cho J.G., Kang H.J., Chae S.W., Hwang S.J., Jung K.Y., Woo J.S. Expression of oncostatin M in chronic obstructive sialadenitis of the submandibular gland // Ann. Otol. Rhinol. Laryngol. 2008. V. 117. № 5. P. 347 - 352.

[11] Cauli A., Yanni G., Pitzalis C., Challacombe S., Panayi G.S. Cytokine and adhesion molecule expression in the minor salivary glands of patients with Sjögren\'s syndrome and chronic sialoadenitis // Ann. Rheum. Dis. 1995. V. 54. № 3. P. 209 - 215.

[12] David T. Wong. Salivary Diagnostics // Wiley-Blackwell. 2008. P. 167 - 174.