Analysis of Proliferative Activity and Apoptosis Hyperplastic Processes of the Cervix

We have quantified by RT-PCR the expression rate of BCL-2, as gene that possess antiapoptotyc activity, and Ki-67 as known proliferation marker, in normal endometrium, and in CIN and squamous cell cervical carcinoma cases. 60 women have been included in studied group: 16 with morphologically proved cervical cancer, 40 with different degree of CIN (cervical intraepithelial neoplasia) and 6 women with normal endometrium that formed the control group. Analysis of BCL-2 mRNA expression showed progradiate decrease from normal epithelium to CIN and CC, where the lowest rate of expression was detected, but in a case of HPV associated CC BCL2 expression increased. On the other hand the opposite results have been received during Ki-67 expression analysis: mRNA expression rate increased along with degree of CIN achieving maximum in cancer cells. These results were correlated with HPV infection in a case of CIN, but in squamous cell carcinoma HPV associated cells has lower proliferative activity. Pathological endometrial proliferation is associated with stimulation of cell proliferative activity (increasing of Ki-67 expression) with simultaneous apoptosis activation (BCL-2 decreasing). This valid for free and HPV infected cells in a case of CIN. But inverse vector of BCL-2 and Ki-67 expression in a case of HPV associated squamous cell carcinoma cells may be explained by modification of virus activity.