Affinity preparation and insulin-binding human blood serum protein diversity investigation

With the help of affinity adsorbent, insulin - binding serum blood components of healthy donors and diabetics were prepared. It is shown that electrophoreticaly diverse lipocalin similar glycoproteins form the complex with insulin in diabetic-s and healthy serums. Insulin-binding component composition is different in diabetic and normal serums. In normal serum albumin and α-fetoprotein with known immunomodulating properties take part in insulin-binding complex, in diabetic complex α1-acid glycoprotein presents. In diabetic serums in the electrophoresis spectrums of insulin-binding glycoprotein α-globulin-s strip increases.

It is supposed that in human blood insulin-binding proteins form hormone transporting complex which includes hydrophobic and hydrophilic hormones and other physiology active substances.