Effect of nitrogen monoxide on testis and its epididimis in experimental inflammation of the animals

А.В. Есипов; А.Г. Мелконян; Г.Г. Мелконян

Effect of nitrogen monoxide on testis and its epididimis in experimental inflammation of the animals

А.В. Есипов¹, А.Г. Мелконян², Г.Г. Мелконян³

research article Pages: 32-38

¹ заслуженный врач РФ, д.м.н., полковник медицинской службы, начальник филиала №1 ФГБУ «3 ЦВКГ им. А.А. Вишневского» (г. Красногорск, Моск. обл.)
² майор медицинской службы, ст. ординатор урологического отделения урологического центра, ФГБУ «3 ЦВКГ им. А.А. Вишневского»
³ майор медицинской службы, ст. ординатор хирургического отделения 301 окружного военного клинического госпиталя (г. Хабаровск). В.К. Шишло - к.м.н., доцент, вед. науч. сотрудник, отдел оперативной хирургии и клинической лимфологии, ГБОУ ДПО «Российская медицинская академия последипломного образования Росздрава» (Москва)

Keywords: nitrogen monoxide experimental inflammation acute testicular inflammation epididymitis lymphotropic therapy proliferative activity mitotic activity stained cells complex lymphotropic and NO-therapy lymphocytes macrophages lymphatic nodes

Abstract

During the experimental inflammation of the testis with an epididimis in white rats, the latter, at different times from the start of treatment were taken out of the experiment an overdose of ether anesthesia. Treatment was the traditional antibiotic therapy, monotherapy exogenous nitrogen monoxide, integrated lymphotropic therapy and exposure to nitrogen monoxide. Were compared a biomaterial of healthy white rats the control group rats with biological the material receiving treatment the above described methods. Testes with epididimis and regional inguinal lymph nodes were collected for immunohistochemical, of electron microscopic, histological investigations. These investigations showed high morphological and microscopic efficiency indicators comprehensive and only a NO - therapies above conventional treatments. As criteria for evaluating the effectiveness of different of therapies, we studied the degree of a proliferative activity of endothelium of lymphatic sinuses, mitotic activity of lymphocytes and macrophages, tissue regeneration interstitial tissue of the epididymis and testis.

How to cite

Есипов А.В., Мелконян А.Г., Мелконян Г.Г. Влияние монооксида азота на семенник и его придаток при экспериментальном воспалении у животных // Технологии живых систем. 2014. № 3. С. 32–38.

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[2] Pekshev A.V., Protasov Yu.S. i dr. Teplofizicheskie kharakteristiki plazmennykh potokov dlya vozdeystviya na biologicheskie tkani // Tezisy dokl. VII Vsesoyuznoy konf. po fizike nizkotemperaturnoy plazmy. Tashkent. 1987. S. 239-240.

[3] Yefimenko N.A., Chernekhovskaya N.Ye., Vyrenkov Yu.Ye. Rukovodstvo po klinicheskoy limfologii. M.: Rossiyskaya meditsinskaya akademiya poslediplomnogo obrazovaniya. 2001. 157 s.

[4] Shekhter A.B., Grachev S.V., Milovanova Z.P., Rudenko T.G. Primenenie ekzogennogo monooksida azota v meditsine: mediko-biologicheskie osnovy, kliniko-morfologicheskie aspekty, mekhanizmy, problemy i perspektivy // NO-terapiya: teoreticheskie aspekty, klinicheskiy opyt i problemy primeneniya ekzogennogo oksida azota v meditsine / pod red. S.V. Gracheva i A.B. Shekhter. M.: Russkiy vrach 2001. S. 27-35.

[5] Vyrenkov Yu.Ye., Yesipov A.V., Moskalenko V.I., Shishlo V.K. Kompleksnaya NO- i limfaticheskaya terapiya v klinicheskoy praktike. M. 2011. 286 s.

[6] Vyrenkov Yu.Ye., Vtorenko V.I., Shevkhuzhev Z.A. Limfologicheskie metody v khirurgii i intensivnoy terapii. M.: RMAPO. 1997. S. 29.

[7] Lazarev A.B. Lechenie fibroplasticheskoy induratsii polovogo chlena metodikami limfaticheskoy terapii i monooksidom azota // Diss. kand. med. nauk. M. 2011.

[8] Yarema I.V., Yevdokimov V.V., Urtaev B.M., Makarchenko A.I. Limfaticheskaya sistema kak zona vypolneniya immunoreanimatsii // Vestnik limfologii. 2013. № 3. S. 26.

[9] Yarema I.V., Merzhvinskiy I.A., Shishlo V.K. i dr. Metod lekarstvennogo nasyshcheniya limfaticheskoy sistemy // Khirurgiya. 1999. № 1. S.14-16

[10] Karaganov Ya.L., Mironov A.A, Mironov V.A. Limfaticheskiy uzel // V sb.: Atlas skaniruyushchey elektronnoy mikroskopii kletok, tkaney i organov. M.: Meditsina. 1987. S. 181-183.

[11] Severin Ye.S., Muyzhnek Ye.L., Severin S.Ye. Kontseptsiya vtorichnykh messendzherov: ot fundamental'nykh osnov - k klinicheskoy praktike. M.: Izd-vo «Dimitreyd Grafik Grupp». 2005. S. 270-299.

[12] Khem A., Kormak D. Gistologiya. V 5-ti t.: Per. s angl. T. 5. M.:Mir. 1983. 272 s.

[13] Andrologiya. Muzhskoe zdorov'e i disfunktsiya reproduktivnoy sistemy: Per. s angl. / pod red. E Nishlaga, G.M. Bere. M.: OOO «Meditsinskoe informatsionnoe agentstvo». 2005. 554 s.

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