Model biological systems and the method of evaluation of spontaneous and initiated by the oxidative modification of proteins in the pool average weight molecules

N.V. Besruchko; G.K. Rubsov

Model biological systems and the method of evaluation of spontaneous and initiated by the oxidative modification of proteins in the pool average weight molecules

research article Pages: 46-50

¹ д.б.н., доцент, профессор кафедры биохимии, ФГБОУ ВПО «Пензенский государственный университет»
² ассистент, кафедра биохимии, ФГБОУ ВПО «Пензенский государственный университет»

Keywords: model biological systems oxidative modification of proteins and initiate spontaneous method of evaluation the average molecular weight

Abstract

The study of oxidative modification of proteins (OMP) can be performed based on the reaction with 2,4-dinitrophenylhydrazine, is a reagent for carbonyl compounds. The base model of the biological system (MBS) for relevant research can serve yolk lipoproteins in a spontaneous and Fe2+-induced oxidation. Another series of MBS were the bee products (propolis, homogenate drone brood, honey, royal jelly) as a test system, with antioxidant properties. MBS considered adding serum rats as biological environment, sensitive to changes in the conditions of free-radical processes.

Purpose - to justify the biological model system to test the method for assessing spontaneous and initiated by the oxidative modification of proteins in a pool of molecules of average weight.

Justification MBS to test method for assessing spontaneous and initiated by OMP in a pool of MSM conducted in the following series of observations: 1) in the MBS with the addition of yolk lipoprotein and without the addition of yolk lipoproteins in the spontaneous and Fe2+-induced oxidation, and 2) the IBS with and without the addition of serum the blood of rats with spontaneous and Fe2+-induced oxidation, and 3) the IBS with and without the addition of yolk lipoprotein and serum of rats with spontaneous and Fe2+-induced oxidation.

The biochemical characteristics of possible MBS through a comparative evaluation in terms of spontaneous and initiated by OMP in a pool of MSM to support the possible use of their complex. Is set at the analyzed model of biological systems to the conditions of Fe2+-induced oxidation, which showed changes in levels of analyzed tests, compared to spontaneous oxidation.

How to cite

Безручко Н.В., Рубцов Г.К. Модельные биологические системы и метод оценки спонтанной и инициированной окислительной модификации белков в пуле молекул средней массы // Технологии живых систем. 2013. № 5. С. 46–50.

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[1] Dubinina E.E., Burmistrov S.O., KHodov D.A., Porotov I.G. Okislitelnaja modifikacija belkov syvorotki krovi cheloveka, metod ee opredelenija// Voprosy medicinskojj khimii. 1995. № 1. S. 24 - 26.

[2] Zajjcev V.G.Modelnye sistemy perekisnogo okislenija lipidov i ikh primenenie dlja ocenki antioksidantnogo dejjstvija lekarstvennykh preparatov: Avtoref. diss. - kand. biol. nauk. Volgograd, 2001. 23 s.

[3] Klebanov G.I., Babenkova I.V., Teselkin JU.O., Komarov O.S., Vladimirov JU.A. Ocenka antiokislitelnojj aktivnosti plazmy krovi s primeneniem zheltochnykh lipoproteidov // Laboratornoe delo. 1988. № 5. S. 59 - 62.

[4] Muravleva L.E., Molotov-Luchanskijj V.B., Kljuev D.A., Bakenova R.A., Kultanov B.ZH., Tankibaeva N.A., Kojjkov V.V., Omarova G.A. Okislitelnaja modifikacija belkov: problemy i perspektivy issledovanija // Fundamentalnye issledovanija. 2010. № 1. S. 74 - 78.

[5] Rubcov G.K., Bezruchko N.V., Sadovnikova D.G., Kozlova G.A., Anopin K.D. Kliniko-biokhimicheskoe znachenie kompleksnogo izuchenija molekul srednejj massy i okislitelnojj modifikacii belkov dlja ocenki ehndotoksikoza // Voprosy biologicheskojj, medicinskojj i farmacevticheskojj khimii. 2013. № 2. S. 41 - 47.

[6] Rubcov G.K., Bezruchko N.V., Gengin M.T., Vasilkov V.G., Borisova E.JU., Anopin K.D., Vasileva A.D., Sadovnikova D.G., Kozlova G.A., Kruchinina A.D., Gamzin S.S. Sposob opredelenija okislitelnojj modifikacii belkov v pule veshhestv srednejj molekuljarnojj massy v syvorotke krovi, plazme, ehritrocitakh i v moche. Zajavka na patent na izobretenie Rossijjskojj Federacii № 2012153044, prioritet ot 7.12.2012.

[7] Frolova M.JU. Vozmozhnosti metoda opredelenija karbonilnykh grupp belkov syvorotki krovi dlja ocenki sostojanija «okislitelnogo stressa» v klinicheskojj praktike: Avtoref. diss. ... kand. med. nauk. SPb. 2003. 25 s.