Article or book title, author's surname, DOI

INFLUENCE OF THE HYPEROKSICHESKY GAS ENVIRONMENT ON EMBRYONIC DEVELOPMENT JAPANESE QUAIL

T.S. Gureva1, O.A. Dadasheva2, P.E. Soldatov3, A.M. Nosovskij4, N.V. Savina5

research article Pages: 56-60

  1. 1 к.б.н., Учреждение РАН Государственный научный центр РФ Институт медико-биологических проблем (ГНЦ РФ ИМБП) РАН (Москва)
  2. 2 ст. науч. сотрудник, Учреждение РАН ГНЦ РФ ИМБП РАН
  3. 3 д.т.н., Учреждение РАН ГНЦ РФ ИМБП РАН
  4. 4 д.б.н., Учреждение РАН ГНЦ РФ ИМБП РАН
  5. 5 науч. сотрудник, Учреждение РАН ГНЦ РФ ИМБП РАН

Abstract

Embrionalnoe development of birds in its various aspects represents a great interest for many re-searchers. It is known that the embryos of birds developing out of a parent organism, actively react to changes of factors of an environment. Berotta, investigating influences of various concentration of oxygen in an incubator (from 14 to 50 %) on a metabolism of embryos of birds, has shown that decrease in exchange processes at germs of birds comes at superfluous oxygen in the environment from 30-40 %

Normal growth of an embryo and morphogenesis birds is impossible without oxygen. The ox-ygen lack during the period embryonic developments causes different uglinesses whereas about a role of surplus of oxygen in development of birds of an unambiguous answer is not present in a germ.

The given work is devoted an influence estimation hyperoxia the gas environment on early embryonic the Japanese has sung. In experiment for hatching quail eggs used skilled oxygen-nitric (О2 - 35 %, N2 65 %) a gas respiratory mix. As the control served hatching eggs in vitro. Du-ration of experiment has made 4 days. Influence of gas environments on early embryonic devel-opment of the Japanese has sung estimated by results hatching, to stages of development of em-bryos, and also a morphological condition of a germ and its bodies, a condition of vessels vitelline a bag. The analysis received embryonic a material after end of experiments has shown that germs in the skilled gas environment though were live at fixing, but were at various stages of the devel-opment from 2,5 till 3,5 days.

Temporary surplus of oxygen in the environment of development перепелиных eggs did not cause destruction of embryos. Research of blood vessels желточного a bag at embryos of the Japanese has sung, developed in hyperoxia and normoxia, has shown that diameter large vessels of 1 and 2 usages at skilled embryos was less, than at control germs. It can lead to easing of pro-cesses erythropoiesis and synthesis of haemoglobin at germs of birds.

Knowing that for incubation quail eggs in hypoxic the gas environment entered 55 % of inert gas of argon that led to positive results, namely, to decrease in destruction an embryo and reduc-tion of anomalies in their development. Therefore, experiment on incubation eggs in oxygen-argono-nitric gas mixes (О2 35 %, N2 10 %, Ar 55 %) has been made. Results have shown that incubation eggs in a gas mix with introduction Ar-55 %, has led to acceleration of term of development of a germ for 4 days on 28,4 %. By means of the one-factorial dispersive analysis it has been shown that diameter of vessels of 1 order: (D1 the beginning of vessels and D1 branch of vessels) and 2 order at the embryos which have developed in hypoxic an oxygen-argono-nitric gas mix is authentic more than at germs, incubate in the oxygen-nitric environment.

How to cite

Гурьева Т.С., Дадашева О.А., Солдатов П.Э., Носовский А.М., Савина Н.В. ВЛИЯНИЕ ГИПЕРОКСИЧЕСКОЙ ГАЗОВОЙ СРЕДЫ НА ЭМБРИОНАЛЬНОЕ РАЗВИТИЕ ЯПОНСКОГО ПЕРЕПЕЛА // Технологии живых систем. 2011. № 4. С. 56–60.

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