EFFECT OF HYDROGEN PEROXIDE ON THE TONE OF RAT ARTERIAL FRAGMENT AND ITS ANTIOXIDANT PROTECTION WITH DERIVATIVES OF CATALASE AND SUPEROXIDE DISMUTASE

A.V. Vavaev; E.G. Tischenko; V.P. Mokh; A.V. Maksimenko

EFFECT OF HYDROGEN PEROXIDE ON THE TONE OF RAT ARTERIAL FRAGMENT AND ITS ANTIOXIDANT PROTECTION WITH DERIVATIVES OF CATALASE AND SUPEROXIDE DISMUTASE

A.V. Vavaev¹, E.G. Tischenko², V.P. Mokh³, A.V. Maksimenko⁴

research article Pages: 36-32

¹ лаборатория биохимической инженерии, Институт экспериментальной кардиологии (ИЭК) Российский кардиологический научно-производственный комплекс (РКНПК) Росмедтехнологий (Москва)
^2,4 лаборатория биохимической инженерии, ИЭК РКНПК Росмедтехнологий
³ лаборатория электрофизиологии миокарда, ИЭК РКНПК Росмедтехнологий

Keywords: aortic rat ring hydrogen peroxide oxidative stress vessel tone superoxide dismutase catalase bienzymic superoxide dismutase - chondroitin sulfate - catalase conjugate antioxidant enzyme therapy

Abstract

Changes in aortic rat ring tone were studied in vitro after addition of varied concentration of hydrogen peroxide and superoxide dismutase (SOD), catalase (CAT) or SOD-chondroitin sulfat-CAT (SOD-CHS-CAT), which were added 10 min before H2O2 at the background of noradrenalin (NA) preconstriction. The concentration-dependent effect of the arterial segment (at 0,01 - 1,0 mM H2O2) caused a constriction, which was superseded by dilatation (at concentration higher than 0,1 mM). High concentration of H2O2 caused irreversible disturbances of vascular activity. 1mM H2O2 was significantly reduced further contractile NA-effect even after multifold wash. Native CAT or SOD-CHS-CAT (at the same enzyme activity concentration) eliminated dose-dependently the effect of H2O2 and promoted preserving of second NA-response. The SOD-CHS-CAT (at 500 E/ml) was more effective than CAT. This effect could relate with experimental proved effect of SOD being part of SOD-CHS-CAT. At the similar conditions native SOD and SOD-CHS-CAT induced the vascular dilatation on 20% due to the increase of NO-bioavailability. SOD-CHS-CAT conjugate possessed in the sum by effects of SOD and CAT simultaneously. This is important for combined antioxidant protection against oxidative stress in vascular.

How to cite

Ваваев А.В., Тищенко Е.Г., Мох В.П., Максименко А.В. Влияние перекиси водорода на тонус артериального фрагмента сосуда крысы и его антиоксидантная защита производными каталазы и супероксиддисмутазы // Технологии живых систем. 2009. № 3. С. 36–32.

References

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[1] Laurindo F.R., da Luz P.L., Uint L., Rocha T.F., Jaeger R.G. Lopez E.A. Evidence for superoxide radical-dependent coronary vasospasm after angioplasty in intact dogs // Circulation. 1991. V. 83. P. 1705-1715.

[2] Leite P.F., Danilovic A., Moriel P., Dantas K., Marklund S., Dantas A.P.V., Laurindo F.R. Sustained disease in superoxide dismutase activity underlies constrictive remodeling after balloon injury in rabbits // Arterioscler. Thromb. Vasc. Biol. 2003. V. 23. P. 2197-2202.

[3] Brandes R.P. Out of balance. A role of impaired superoxide dismutase activity for vascular constrictive remodeling after angioplasty // Arterioscler. Thromb. Vasc. Biol. 2003. V. 23.
P. 2121 - 2122.

[4] Maksimenko A.V. Experimental antioxidant biotherapy for protection of the vascular wall by modified forms of superoxide dismutase and catalase // Curr. Pharm. Design, 2005. V. 11. P. 2007-2016.

[5] Maksimenko A.V., Golubykh V.L., Tischenko E.G. The combination of modified antioxidant enzymes for anti-thrombotic protection of the vascular wall: the significance of covalent connection of superoxide dismutase and catalase activities // J. Pharmacol. 2004. V. 56. P. 1463-1468.

[6] Halliwell B., Clement M.V., Long L.H. Hydrogen peroxide in the human body // FEBS Letters. 2000. V. 486. P. 10-13.

[7] Faraci F.M. Hydrogen peroxide. Watery fuel for change in vascular biology // Arterioscler. Thromb. Vasc. Biol. 2006. V. 26. P. 1931-1933

[8] Thengchaisri N., Hein T.W., Wang W., Xu X., Li Z., Fossum T.W., Kuo L. Upregulation of arginase by H₂O₂impairs endothelium-dependent nitric oxide-mediated dilatation of coronary arterioles // Arterioscler. Thromb. Vasc. Biol. 2006. V. 26. P. 2035-2042.

[9] Walia M., Sormaz L., Samsom S.E., Lee R.M., Grower A.K. Effect of hydrogen peroxide on pig coronary artery endothelium // Eur. J. Pharmacol. 2000. V. 400. P. 249-253.

[10] Максименко А.В., Тищенко Е.Г. Ваваев А.В., Петрова М.Л. Сочетанное действие антиоксидантных ферментных производных супероксиддисмутазы и каталазы для антитромботической защиты сосудистой стенки // Здоровье. Медицинская экология. Наука. Дальневосточный медицинский научно-практический журнал. 2005. №1(21). C. 10-13.

[11] Maiellaro K., Taylor W.R. The role of the adventitia in vascular inflammation // Cardiovasc. Res. 2007. V. 75. P. 640-648.

[12] Stone J.R., Yang S. Hydrogen peroxide: a signaling messenger // Antioxid. Redox. Signal. 2006. V. 8. P. 243-280.

[13] Максименко А.В. Внеклеточное оксидативное поражение сосудистой стенки и ее ферментная антиоксидантная защита // Химико-фармацевтический журнал. 2007. №5(41). С. 3-12.

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